摘要
目的:探讨大蒜素对肥胖伴牙周炎大鼠胰岛素抵抗及游离脂肪酸(free fatty acid,FFAs)水平的影响。方法:40只大鼠随机分为健康组、牙周炎组及低、中、高剂量组,每组8只。健康组为健康大鼠,其余各组均以谷氨酸钠(MSG)诱法,成功建立肥胖模型后,结扎上颌磨牙并涂菌,建立牙周炎模型。牙周炎组和健康组均给予生理盐水,低、中、高剂量组分别给予大蒜素20、40、60 mg·kg^(-1)·d^(-1),灌胃给药。治疗21天后,检测大鼠空腹血糖(FPG)、空腹胰岛素(FINS),稳态模型的胰岛素抵抗指数(HOMA-IR)评分及FFAs水平,比较TLR4/MyD88信号通路的蛋白表达。采用SPSS 22.0软件包对数据进行统计学分析。结果:与健康组相比,牙周炎组的FPG、FINS水平,HOMA-IR,IL-6及TNF-α水平显著升高,TLR4、MyD88蛋白表达显著上调(P<0.05)。与牙周炎组相比,低、中、高剂量的FPG、FINS水平,HOMA-IR,IL-6及TNF-α水平显著降低,TLR4、My D88蛋白表达显著下调(P<0.05)。与低剂量组相比,中、高剂量组FPG、FINS水平,HOMA-IR,IL-6及TNF-α水平显著降低,TLR4、MyD88蛋白表达显著下调(P<0.05)。与中剂量组相比,高剂量组FPG、FINS水平,HOMA-IR,IL-6及TNF-α水平显著降低,TLR4、MyD88蛋白表达显著下调(P<0.05)。低、中、高剂量组治疗后的FFAs显著低于治疗前(P<0.05)。治疗后,与健康组相比,牙周炎组、低、中剂量组FFAs水平显著升高。与牙周炎组相比,低、中、高剂量组FFAs水平显著降低;与低剂量组相比,高剂量组FFAs水平显著降低;与中剂量组相比,高剂量组FFAs水平显著降低(P<0.05)。结论:大蒜素能改善肥胖伴牙周炎大鼠胰岛素抵抗和肥胖情况,其作用机制与TLR4/MyD88信号通路有关。
PURPOSE:To explore the effects of allicin on insulin resistance and free fatty acids(FFAs)levels in obese rats with periodontitis.METHODS:Forty rats were randomly divided into healthy group,periodontitis group,and low,medium and high dose groups,with 8 rats in each group.The healthy group was healthy rats,and the other groups were induced by sodium glutamate(MSG).After successfully establishing an obesity model,the maxillary molars were ligated and smeared to establish a periodontitis model.Both the periodontitis group and the healthy group were given normal saline,and the allicin low,medium and high dose groups were given allicin 20,40 and 60 mg·kg-1·d-1,mixed with feed for oral administration.After 21 days of treatment,the fasting blood glucose(FPG),fasting insulin(FINS),insulin resistance index(HOMA-IR)scores and FFAs levels of the homeostatic model in rats were detected.The protein expression of TLR4/MyD88 signaling pathway were compared.Statistical analysis was performed with SPSS 22.0 software package.RESULTS:Compared with the healthy group,FPG,FINS levels,HOMA-IR,IL-6 and TNF-αlevels of the periodontitis group were significantly increased,and the expression of TLR4 and MyD88 proteins was significantly increased(P<0.05).Compared with the periodontitis group,FPG,FINS levels,HOMA-IR,IL-6 and TNF-αlevels of low,medium and high-doses groups were significantly decreased,and the expression of TLR4 and MyD88 proteins was significantly decreased(P<0.05).Compared with the low-dose group,the levels of FPG and FINS,HOMA-IR,IL-6 and TNF-αlevels of the middle and high-dose groups were significantly decreased,and the expression of TLR4 and MyD88 proteins was significantly decreased(P<0.05).Compared with the middle-dose group,the levels of FPG and FINS,HOMA-IR,IL-6 and TNF-αlevels of the high-dose group were significantly decreased,and the expression of TLR4 and MyD88 proteins was significantly decreased(P<0.05).After treatment,FFAs of the low,medium and high-dose groups were significantly lower than those before treatment(P<0.05).Compared with the healthy group,FFAs levels of the periodontitis group,low-dose and medium-dose groups were significantly increased.Compared with the periodontitis group,FFAs levels of the low,medium and high-dose groups were significantly increased.Compared with the low-dose group,FFAs levels of the high-dose group were significantly increased.Compared with the middle-dose group,FFAs levels of the high-dose group were significantly increased(P<0.05).CONCLUSIONS:Allicin can improve insulin resistance and obesity in obese rats with periodontitis,and its mechanism of action is related to the TLR4/MyD88 signaling pathway.
作者
谢兰芬
李晓静
黄晓霖
赵升柯
XIE Lan-fen;LI Xiao-jing;HUANG Xiao-lin;ZHAO Sheng-ke(Department of Stomatology,The First Affiliated Hospital of Guizhou University of Chinese Medicine.Guiyang 550000,Guizhou Province,China)
出处
《上海口腔医学》
CAS
北大核心
2023年第4期375-379,共5页
Shanghai Journal of Stomatology
基金
贵州省科技计划项目(黔科合[2018]2754号)。
