摘要
目的分析探讨CD133^(+)结肠癌细胞3-UTR区域rs2240688变异位点在结肠癌免疫逃逸中的作用。方法检测CD133^(+)结肠癌细胞3-UTR区域rs2240688位点单核苷酸多态性,并分别检测不同基因型CD133^(+)结肠癌细胞侵袭迁移能力以及NK-92细胞对不同基因型CD133^(+)结肠癌细胞的细胞毒力。结果基因型为G/G的CD133^(+)结肠癌细胞侵袭迁移能力最强,而基因型为T/T的CD133^(+)结肠癌细胞侵袭迁移能力最弱(P均<0.05);NK-92细胞对基因型为G/G的CD133^(+)结肠癌细胞的细胞毒力最弱,而对基因型为T/T的CD133^(+)结肠癌细胞的细胞毒力最强(P均<0.05)。结论CD133^(+)结肠癌细胞3-UTR区域单核苷酸多态性位点基因多态性能够参与机体的免疫逃逸,对CD133^(+)肿瘤干细胞进行深入研究,有望成为结直肠癌临床治疗的新靶点。
Objective To investigate the role of rs2240688 mutation in CD133^(+) colon cancer cell line 3-UTR region in immune escape from colon cancer.Methods Single nucleotide polymorphism at rs2240688 in the 3-UTR region of CD133^(+) colon cancer cells was detected,and the invasion and migration ability of CD133^(+) colon cancer cells and the cytotoxicity of NK-92 cells to CD133^(+) colon cancer cells of different genotypes were detected.Results CD133^(+) colon cancer cells with G/G genotype had the strongest invasion and migration ability,while CD133^(+) colon cancer cells with T/T genotype had the weakest invasion and migration ability(all P<0.05).The cytotoxicity of NK-92 cells to CD133^(+) colon cancer cells with G/G genotype was the weakest,while the cytotoxicity of NK-92 cells to CD133^(+) colon cancer cells with T/T genotype was the strongest(all P<0.05).Conclusions The gene polymorphism of single nucleotide polymorphism locus in the 3-UTR region of CD133^(+) colon cancer cells can participate in immune escape of the body,and CD133^(+) tumor stem cells are expected to be further studied and become a new target for clinical treatment of colorectal cancer.
作者
李一鹤
钱树坤
刘建华
LI Yihe;QIAN Shukun;LIU Jianhua(Department of Clinical Laboratory,Beijing Rehabilitation Hospital Affiliated to Capital Medical University,Beijing 100041,China)
出处
《中国肿瘤外科杂志》
CAS
2023年第4期345-350,共6页
Chinese Journal of Surgical Oncology
