摘要
目的探讨与分析间充质干细胞(Mesenchymal stem cells,MSCs)介导核苷酸结合寡聚化结构域样受体蛋白3(Nucleotide-binding oligomeric domain-like receptor protein 3,NLRP3)/半胱氨酸天冬氨酸蛋白水解酶-1(Cysteinerequiring aspartate protease-1,Caspase-1)通路对骨关节炎模型大鼠软骨细胞凋亡的影响。方法将骨关节炎模型大鼠42只随机分为3组:模型组、地塞米松组与MSCs组,每组14只。模型组给予生理盐水5mL/kg灌胃,每周2次;地塞米松组给予肌肉注射20mg/kg地塞米松约200μL,每周2次;MSCs组给予MSCs注射治疗,每周2次。3组分别在治疗第4周、治疗第8周各处死7只大鼠,检测软骨细胞凋亡情况。结果地塞米松组与MSCs组治疗第4周、第8周的血清碱性磷酸酶(ALP)含量、软骨细胞凋亡指数水平、软骨组织NLRP3和Caspase-1蛋白相对表达水平均低于模型组(P<0.05),且MSCs组低于地塞米松组(P<0.05)。地塞米松组与MSCs组治疗第4周、第8周的股骨、腰椎骨密度高于模型组(P<0.05),且MSCs组高于地塞米松组(P<0.05)。结论在骨关节炎模型大鼠中应用MSCs能降低血清ALP含量,提高股骨、腰椎骨密度,还可抑制软骨组织NLRP3、Caspase-1蛋白的表达,降低软骨细胞凋亡水平。
Objective To investigate and analyse the effects of mesenchymal stem cells(MSCs)mediated nucleotide-binding oligomeric domain-like receptor protein 3(NLRP3)/cysteine-requiring aspartate protease-1(Caspase-1)pathway on chondrocyte apoptosis in rats with osteoarthritis.Methods 42 cases of osteoarthritis model rats were randomly divided into three groups:model group,dexamethasone group and MSCs group,with 14 rats in each group.The model group was given physiological saline by gavage,5mL/kg,twice a week.The dexamethasone group was given intramuscular injection of 20mg/kg dexamethasone injection of about 200μL,twice a week.MSCs group was treated with injection of MSCs,twice a week.Seven rats were killed in each of the three groups at the 4th and 8th weeks of treatment,respectively,and the chondrocytes apoptosis were detected.Results The serum alkaline phosphatase(ALP)content,chondrocyte apoptosis index and the relative expression levels of NLRP3 and Caspase-1 protein in cartilage tissue in the dexamethasone group and MSCs group were lower than those in the model group at the 4th and 8th weeks of treatment(P<0.05),and the above indicators in the MSCs group were significantly lower than those in the dexamethasone group(P<0.05).The bone mineral density of the femur and lumbar vertebrae in the dexamethasone group and the MSCs group were significantly higher than those in the model group at the 4th and 8th weeks of treatment(P<0.05),and the MSCs group was significantly higher than the dexamethasone group(P<0.05).Conclusion The application of MSCs in osteoarthritis model rats can reduce the content of serum ALP,increase bone mineral density of femur and lumbar spine,inhibit the expression of NLRP3 and Caspase-1 protein in cartilage tissue,and reduce the level of chondrocyte apoptosis.
作者
王海
于涛
Wang Hai;Yu Tao(Department of Orthopedics,the Second Hospital of Mudanjiang Medical College,Mudanjiang 157000)
出处
《中国现代医药杂志》
2023年第7期1-5,共5页
Modern Medicine Journal of China
基金
黑龙江省卫生健康委科研课题(编号:2020-397)。
