摘要
目的观察隔药饼灸对高脂血症兔肝组织Wnt1、β-卷曲蛋白(β-catenin)、散乱蛋白-1(dishevelled-1,dvl-1)、固醇调节元件结合蛋白1c(sterol regulatory element-binding protein1c,SREBP1c)的mRNA表达量的影响,探讨隔药饼灸调脂的作用机制。方法将48只新西兰兔随机分为正常组、模型组、隔药饼灸组和阿托伐他汀组,每组12只。正常组采用普通饲料喂养,其余各组采用高脂饲养法喂养8周,待造模成功后,除正常组、模型组外,隔药饼灸组选取两组穴位交替施灸(Ⅰ组穴位为巨阙、天枢、丰隆;Ⅱ组穴位为心俞、肝俞、脾俞),阿托伐他汀组将阿托伐他汀钙片碾成粉末后拌入第一口饲料喂食,每日1次,连续8周。测定各组兔血清总胆固醇(total cholesterol,TC)、甘油三酯(glycerin trilaurate,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)含量,RT-PCR法测定各组兔Wnt1、β-catenin、dvl-1、SREBP1c的mRNA表达量,油红O染色观察肝组织脂质沉积情况。结果(1)血脂结果:与正常组相比,模型组血清TC、TG、LDL-C上升(P<0.001);与模型组相比,隔药饼灸组和阿托伐他汀组TC、TG、LDL-C下降(P<0.01,P<0.001);隔药饼灸组与阿托伐他汀组相比,TC、TG、LDL-C差异无统计学意义(P>0.05)。(2)RT-PCR结果:与正常组相比,模型组肝组织Wnt1、β-catenin、dvl-1、SREBP1c的mRNA表达量上升(P<0.001);与模型组相比,隔药饼灸组和阿托伐他汀组肝组织Wnt1、β-catenin、dvl-1、SREBP1c的mRNA表达量均下降(P<0.01,P<0.001);隔药饼灸组与阿托伐他汀组肝组织Wnt1、β-catenin、dvl-1、SREBP1c的mRNA表达差异无统计学意义(P>0.05)。(3)脂质沉积结果:与正常组相比,模型组、隔药饼灸组、阿托伐他汀组脂质沉积均有增加,模型组增加更明显;与模型组相比,隔药饼灸组、阿托伐他汀组脂质沉积明显减少;隔药饼灸组与阿托伐他汀组无明显差异。结论隔药饼灸可能通过抑制Wnt/β-catenin通路,下调高脂血症兔SREBP1c含量,发挥调脂作用。
Objective To observe the effects of medicinal cake-separated moxibustion on Wnt1,β-catenin,dishevelled-1(dvl-1),and sterol regulatory element-binding protein1c(SREBP1c)in liver tissue of hyperlipidemia rabbits and to explore the mechanism of action of medicinal cake-separated moxibustion on lipid regulating.Methods A total of 48 New Zealand rabbits were randomly divided into normal group,model group,medicinal cake-separated moxibustion group,and atorvastatin group,with 12 rabbits in each group.The normal group was fed with normal diet,while the other groups were fed with high-fat diet for 8 weeks.After successful modeling,except normal group and model group,two groups of acupoints in medicinal cake-separated moxibustion group were selected for alternating moxibustion[groupⅠ:'Juque'(RN14),'Tianshu'(ST25),and'Fenglong'(ST40);groupⅡ:‘Xinshu'(BL15),'Ganshu'(BL18),and'Pishu'(BL20)];in atorvastatin group,atorvastatin calcium tablets were ground into powder and mixed into the first feed,once a day for 8 consecutive weeks in both groups.The content of total cholesterol(TC),glycerin trilaurate(TG),and low density lipoprotein cholesterol(LDL-C)in serum of rabbits in each group were determined.The mRNA expression levels of Wnt1,β-catenin,dvl-1 and SREBP1c were determined by RT-PCR.Lipid deposition in liver tissue was observed by oil red"O"staining.Results(1)Lipid results:Compared with normal group,serum TC,TG,and LDL-C in model group increased(P<0.001);compared with model group,TC,TG,and LDL-C in medicinal cake-separated moxibustion group and atorvastatin group decreased(P<0.01,P<0.001);compared with atorvastatin group,there was no significant statistical difference in TC,TG,and LDL-C in medicinal cake-separated moxibustion group(P>0.05).(2)RT-PCR results:Compared with normal group,mRNA expression levels of Wnt1,β-catenin,dvl-1,and SREBP1c in liver tissue in model group increased(P<0.001);compared with model group,mRNA expression levels of Wnt1,β-catenin,dvl-1,and SREBP1c in liver tissue in both medicinal cake-separated moxibustion group and atorvastatin group decreased(P<0.01,P<0.001).There was no significant statistical difference in mRNA expression levels of Wnt1,β-catenin,dvl-1,and SREBP1c in liver tissue between medicinal cake-separated moxibustion group and atorvastatin group(P>0.05).(3)Lipid deposition results:Compared with normal group,lipid deposition in model group,medicinal cake-separated moxibustion group,and atorvastatin group increased,with more significant increase in model group;compared with model group,lipid deposition in medicinal cake-separated moxibustion group and atorvastatin group reduced significantly,and there was no significant difference between medicinal cake-separated moxibustion group and atorvastatin group.Conclusion By inhibting Wnt/β-catenin pathway,medicinal cake-separated moxibustion can down-regulate the content of SREBP1c and play the role of lipid regulation.
作者
刘惠娟
欧阳里知
李芊
彭涵
刘红华
葛君芸
罗坚
常小荣
刘迈兰
汪厚莲
LIU Huijuan;OUYANG Lizhi;LI Qian;PENG Han;LIU Honghua;GE Junyun;LUO Jian;CHANG Xiaorong;LIU Mailan;WANG Houlian(Hunan College of Chinese Medicine,Zhuzhou,Hunan 412012,China;Hunan University of Chinese Medicine,Changsha,Hunan 410208,China)
出处
《湖南中医药大学学报》
CAS
2023年第6期1098-1104,共7页
Journal of Hunan University of Chinese Medicine
基金
国家自然科学基金项目(82074559)
湖南省研究生科研创新项目(CX20200781)
长沙市杰出创新青年培养计划项目(kq1905036)
湖南省教育厅优秀青年基金项目(19B435,19B428)
湖南中医药大学第一附属医院湖南省院士专家工作站(石学敏)开放基金(2019YSZJJ)
湖南省中医药科研计划课题项目(201964)。
