摘要
目的探讨浙江地区健康儿童血清胆汁酸谱特征。方法横断面研究,选取2020年1月至2022年7月在浙江大学医学院附属儿童医院进行体检的影像学资料和实验室生化指标正常的245名健康儿童为研究对象。采集隔夜空腹静脉血,应用串联质谱法准确定量血清中18种胆汁酸浓度。比较不同性别组胆汁酸亚型浓度差异并探讨年龄与胆汁酸水平的相关性。采用Mann-Whitney U检验进行组间比较,Spearman检验进行相关性分析。结果245名健康儿童中男125名、女120名,年龄10(8,12)岁。不同性别组间血清总胆汁酸、初级与次级胆汁酸、游离型与结合型胆汁酸浓度的差异均无统计学意义(均P>0.05)。女童组血清熊脱氧胆酸和甘氨熊脱氧胆酸浓度均明显高于男童组[199.0(66.9,276.5)比154.7(49.3,205.0)nmol/L,274.0(64.8,308.0)比181.0(43.8,209.3)nmol/L,Z=2.06、2.71,均P<0.05]。男童组与女童组血清牛磺石胆酸均与年龄呈正相关(r=0.31、0.32,均P<0.05)。男童组血清鹅脱氧胆酸、甘氨鹅脱氧胆酸均与年龄呈正相关(r=0.20、0.23,均P<0.05);女童组血清牛磺熊脱氧胆酸与年龄呈负相关(r=-0.27,P<0.05),胆酸与年龄呈正相关(r=0.34,P<0.05)。结论浙江地区健康儿童人群总胆酸水平相对稳态,但是胆汁酸亚型表现出性别差异,并与年龄呈一定的相关性。
Objective To characterize the serum bile acid profiles of healthy children in Zhejiang Province.Methods A cross-sectional study was conducted on 245 healthy children who underwent imaging and laboratory biochemical tests during routine physical examinations at the Children′s Hospital of Zhejiang University School of Medicine from January 2020 to July 2022.Overnight fasting venous blood samples were collected,and the concentrations of 18 individual bile acids in the serum were accurately quantitated using tandem mass spectrometry.The concentration difference of bile acid were compared between different genders and to explore the correlation between age and bile acid levels.Used the Mann-Whitney U test for intergroup comparison and Spearman test to correlation analysis.Results A total of 245 health children with a age of 10(8,12)years including 125 boys and 120 girls.There were no significant differences in levels of total bile acids,primary and secondary bile acids,free and conjugated bile acids between the two gender groups(all P>0.05).The serum concentrations of ursodeoxycholic acid and glycoursodeoxycholic acid in girls were significantly higher than those in boys(199.0(66.9,276.5)vs.154.7(49.3,205.0)nmol/L,274.0(64.8,308.0)vs.181.0(43.8,209.3)nmol/L,Z=2.06,2.71,both P<0.05).The serum taurolithocholic acid in both boys and girls were positively correlated with age(r=0.31,0.32,both P<0.05).The serum chenodeoxycholic acid and glycochenodeoxycholic acid in the boys group were positively correlated with age(r=0.20,0.23,both P<0.05),whereas the serum tauroursodeoxycholic acid in the girls group was negatively correlated with age(r=-0.27,P<0.05),and the serum cholic acid was positively correlated with age(r=0.34,P<0.05).Conclusions The total bile acid levels are relatively stable in healthy children in Zhejiang province.However,individual bile acids showed gender differences and were correlated with age.
作者
刘阿娜
沈红强
徐翠芳
蒋玲
邵洁
舒强
傅君芬
倪艳
Liu Ana;Shen Hongqiang;Xu Cuifang;Jiang Ling;Shao Jie;Shu Qiang;Fu Junfen;Ni Yan(Department of Clinical Laboratory,Children′s Hospital,Zhejiang University School of Medicine,National Clinical Research Center for Child Health,Hangzhou 310005,China;Department of Child Healthcare,Children′s Hospital,Zhejiang University School of Medicine,National Clinical Research Center for Child Health,Hangzhou 310005,China)
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2023年第6期509-514,共6页
Chinese Journal of Pediatrics
基金
国家自然科学基金(82170583,81900510)
“十四五”重点研发项目(2021YFC2701904)。
关键词
儿童
血清
串联质谱法
胆汁酸
Child
Serum
Tandem mass spectrometry
Bile acids
