摘要
目的探讨柚类黄酮对醋酸铅致小鼠损伤的保护作用。方法将36只小鼠随机分为空白对照组、模型对照组、阳性药物对照组和柚类黄酮干预组(低、中、高剂量分别为100、200、400 mg/mL)。给药4周,计算肝、肾系数,检测小鼠血清中超氧化物歧化酶(SOD)活性以及丙二醛(MDA)含量。结果与空白对照组比较,模型对照组小鼠肝脏系数和肾脏系数升高(P<0.05),SOD活性降低(P<0.05),MDA含量升高(P<0.05),铅中毒造模成功。与模型对照组比较,黄酮化合物低剂量组(100 mg/mL)肾脏系数降低(P<0.05),小鼠血清SOD含量的升幅与MDA的降幅差异无统计学意义(P>0.05);黄酮化合物中剂量组(200 mg/mL)肝脏系数与肾脏系数均降低(P<0.05),且SOD活性提高(P<0.05),MDA含量降低(P<0.05);黄酮化合物高剂量组(400 mg/mL)肾脏系数降低(P<0.05),小鼠血清SOD含量略提高,MDA含量降低,但差异无统计学意义(P>0.05)。结论柚类黄酮可有效减轻醋酸铅对小鼠机体的损伤,但存在最优剂量。
【Objective】To investigate the protective effect of grapefruit flavonoids on lead acetate induced injury in mice.【Methods】A total of 36 mice were randomly divided into blank control group,model group,positive drug control group and pomelo flavonoid intervention group(low,medium and high doses of 100,200 and 400mg/mL,respectively).After 4 weeks of administration,the liver and kidney coefficients were calculated,the activity of superoxide dismutase(SOD)and the content of malondialdehyde(MDA)in serum were detected.【Results】Compared with blank control group,liver coefficient and kidney coefficient in model group were significantly increased(both P<0.05),SOD activity was significantly decreased(P<0.05),MDA content was significantly increased(P<0.05),lead poisoning model was successfully established.Compared with model group,the liver coefficient of 100 mg/mL pomelo flavonoids group was not significantly decreased(P>0.05),but the kidney coefficient was significantly decreased(P<0.05).There was no statistical difference between the increase of SOD content and the decrease of MDA in serum of mice(P>0.05).In 200 mg/mL pomelo flavonoids group,liver coefficient and kidney coefficient were significantly decreased(both P<0.05),SOD activity was significantly increased(P<0.05),MDA content was significantly decreased(P<0.05).In 400 mg/mL pomelo flavonoids group,liver coefficient was not significantly decreased(P>0.05),kidney coefficient was significantly decreased(P<0.05),serum SOD content was slightly increased,but MDA content was decreased,but there was no statistical difference(P>0.05).【Conclusion】Pomelo flavonoids can effectively reduce the damage of lead acetate in mice,but there is an optimal dose.
作者
杨甜甜
朱程滢
谌洁
董俊
YANG Tiantian;ZHU Chengying;CHEN Jie;DONG Jun(The First Clinical College of Changsha Medical University,Changsha,Hunan 410219,China;Department of Physiology,Basic Medical College of Changsha Medical University,Changsha,Hunan 410219,China)
出处
《中国医学工程》
2023年第5期69-72,共4页
China Medical Engineering
基金
湖南省大学生研究性学习和创新性实验计划项目(湘教通〔2020〕191号-3963)。
关键词
柚类黄酮
氧化应激
铅中毒
pomelo flavonoids
oxidative stress
lead poisoning
