摘要
以新鲜牡蛎为原料,通过酶解法制备牡蛎粗多糖,再经Sevage法脱蛋白和Sephadex G⁃200凝胶柱层析分离,浓缩、冷冻干燥后,制得纯化牡蛎多糖.对牡蛎多糖制备中各个阶段的组分进行体外抗氧化活性检测,最后利用傅里叶红外光谱(FTIR)分析纯化牡蛎多糖的化学键和官能团.结果表明,酶解法提取的牡蛎粗多糖得率达到3.69%,纯化操作并不会显著改变牡蛎多糖的抑制羟自由基能力,但纯化牡蛎多糖具有较好的抗超氧阴离子自由基能力(64.97 U·L^(-1)),且含有吡喃糖苷3个特征吸收峰(1154.84、1080.63、1021.93 cm^(-1)),为一种α型吡喃糖,研究结果可为今后牡蛎多糖的制备与应用奠定基础.
In this study,crude oyster polysaccharides were prepared from fresh oysters by enzymatic digestion.After being deproteinized using the Sevage method and separated by Sephadex G⁃200 gel column chromatography,the crude oyster polysaccharides were further concentrated and freeze⁃dried to obtain purified oyster polysaccharides.The fractions at each stage of oyster polysac⁃charides preparation were used to measure antioxidant activity in vitro.The chemical bonds and functional groups of purified oyster polysaccharides were analyzed by Fourier Transform infrared spectroscopy(FTIR).The extraction of oysters by using enzymatic hydrolysis yielded 3.69%crude polysaccharides.The hydroxyl radical inhibiting measurement showed that the oyster polysaccharide purification did not significantly impact the inhibiting ability,while the purified oyster polysaccha⁃rides significantly affected the anti⁃superoxide anion radical ability(64.97 U·L^(-1)).FTIR spec⁃trum showed the polysaccharides contained three characteristic absorption peaks of pyranoside(1154.84,1080.63,1021.93 cm^(-1)),which was anα⁃type pyranose.This study can serve as a foundation for future research on the preparation and application of oyster polysaccharides.
作者
林靖莹
黄姝玲
孟鹏
陈毅斌
吴明玉
黄鹭强
陈慧斌
LIN Jingying;HUANG Shuling;MENG Peng;CHEN Yibin;WU Mingyu;HUANG Luqiang;CHEN Huibin(College of Life Sciences,Fujian Normal University,Fuzhou 350117,China;Fujian Inspection And Research Institute For Product Quality,Fuzhou 350002,China;Marine Active Substance and Product Technology Research and Development Center of Ocean Research Institute of Fuzhou,Fujian Normal University,Fuzhou 350117,China;Key Laboratory of Special Marine Bio-Resources Sustainable Utilization of Fujian Province,College of Life Sciences,Fujian Normal University,Fuzhou 350117,China)
出处
《福建师范大学学报（自然科学版）》
CAS
2023年第3期70-77,共8页
Journal of Fujian Normal University：Natural Science Edition
基金
国家自然科学基金资助项目(31401564)
闽江学者特聘教授计划启动基金资助项目
福建省科技计划高校产学合作项目(2022N5015)
福州海洋研究院科技项目(2022F14)
福建师范大学2022年大学生创新训练计划项目(202210394010)。
关键词
牡蛎多糖
制备工艺
抗氧化活性
oyster polysaccharides
preparation
antioxidant activity
