摘要
构建基于氮掺杂还原氧化石墨烯@羧基化多壁碳纳米管(N-rGO@CMWCNT)和壳聚糖@金纳米颗粒(CS@AuNP)修饰的电化学免疫传感器检测血清中糖类抗原125(CA125)。将N-rGO@CMWCNT和CS@AuNP依次修饰在玻碳电极表面,二者可通过化学作用稳定结合;再将CA125抗体孵育在电极表面,抗体与AuNP形成金硫键(Au—S),可用于检测CA125抗原,采用差分脉冲伏安法(DPV)检测不同浓度CA125所对应的电信号。扫描电子显微镜(SEM)图显示N-rGO@CMWCNT和CS@AuNP的成功合成,Raman光谱表明氮元素成功掺杂于碳纳米复合材料,线性循环伏安法(CV)曲线显示免疫传感器的成功构建。在最优实验条件下,该传感器对CA125检测线性范围为1 mU/mL~100 U/mL,检出限低至0.4 mU/mL,样品的加标回收率在94.5%~107.7%之间,同时其可在10天内保持稳定。该方法精密性好、准确度高、抗干扰能力强,可应用于肺癌患者血清中CA125的检测。
An electrochemical immunosensor based on the modification of nitrogen-doped reduced graphene oxide@carboxylated multi-walled carbon nanotubes(N-rGO@CMWCNTs)and chitosan@gold nanoparticles(CS@AuNPs)was constructed to detect carbohydrate antigen 125(CA125)in serum.The N-rGO@CMWCNTs and CS@AuNPs were modified on the surface of glassy carbon electrode sequentially,and could be stably combined through chemical action.CA125 antibodies were then incubated on the electrode surface.The gold-sulfur(Au—S)bonds can be formed between antibodies and AuNPs to detect CA125 antigens.The electrical signals corresponding to CA125 antigens with different concentrations were detected by differential pulse voltammetry(DPV).Scanning electron microscopy(SEM)images show the successful synthesis of N-rGO@CMWCNTs and CS@AuNPs,Raman spectra indicate that element nitrogen is successfully doped into carbon nanocomposites,and linear cyclic voltammetry(CV)curves show that the immunosensor is built successfully.Under the optimal experimental conditions,the linear range of the sensor for CA125 detection is 1 mU/mL-100 U/mL,and the detection limit is as low as 0.4 mU/mL.The standard recovery rates are in the range of 94.5%-107.7%,and the immunosensor can remain stable for 10 days.The method has good precision,high accuracy and strong anti-interference ability,and can be applied for detecting CA125 in serum from patients with lung cancer.
作者
龚国傲
顾缨缨
王元昊
梅勇
潘洪志
Gong Guoao;Gu Yingying;Wang Yuanhao;Mei Yong;Pan Hongzhi(Key Laboratory of Occupational Hazards Identification and Control in Hubei Province,School of Public Health,Wuhan University of Science and Technology,Wuhan 430065,China;Zhoupu Hospital Affiliated with Shanghai University of Medicine and Health Sciences,Shanghai 201318,China)
出处
《微纳电子技术》
CAS
北大核心
2023年第4期534-541,共8页
Micronanoelectronic Technology
基金
国家自然科学基金(81973097)。
