摘要
为了解河南省假禾谷镰孢菌对氟环唑的敏感性,本文采用菌丝生长速率法测定了氟环唑对2019年从河南省16个地市分离的100株假禾谷镰孢菌的毒力。结果表明:氟环唑对供试菌株菌丝生长的EC_(50)值为0.0024~0.5054μg·mL^(-1),EC_(50)平均值为(0.1093±0.0957)μg·mL^(-1);敏感性频率分布图显示,89株供试菌株位于相应的主峰范围内,敏感性频率分布为连续单峰曲线,将其EC_(50)平均值为(0.0821±0.0468)μg·mL^(-1)作为假禾谷镰孢菌对氟环唑的敏感性基线。方差分析显示:不同地市菌株对氟环唑的敏感性差异较小,各地市菌株EC_(50)平均值变化范围为0.0508~0.2968μg·mL^(-1);同一地市菌株对氟环唑的敏感性差异较大,商丘虞城最不敏感菌株的EC_(50)值是最敏感菌株的210.58倍。聚类分析表明:河南省假禾谷镰孢菌对氟环唑的敏感性差异与菌株的地理来源无明显关联性。氟环唑对假禾谷镰孢菌的毒力与吡唑醚菌酯、咯菌腈、苯醚甲环唑、多菌灵、氰烯菌酯及戊唑醇毒力之间无明显相关性。氟环唑拌种阻碍小麦生长,对其根长、株高及鲜质量均有抑制作用。温室试验中,12.5%氟环唑悬浮剂有效浓度为150μL·mL^(-1)时喷雾防效最高,达到60.18%;离体试验中,治疗性喷雾,不仅有较好的防治效果,且对小麦生长无影响,12.5%氟环唑悬浮剂有效浓度为150μL·mL^(-1)时防效最高,达到79.78%。本研究结果为氟环唑应用于小麦茎基腐病防治提供理论依据,为病原菌对药剂的敏感性监测奠定基础。
In order to detect the sensitivity of Fusarium pseudograminearum to epoxiconazole in Henan Pro-vince,100 isolates were collected from 16 counties in 2019 and their sensitivity to epoxiconazole(EC_(50))were conducted by mycelial growth rate method.The results indicated that the EC_(50)of the tested isolates ranged from 0.0024 to 0.5054μg·mL^(-1),and the average value was(0.1093±0.0957)μg·mL^(-1).The sensitivity frequency distribution showed that EC_(50)values of the 89 tested isolates were lied in a main peak;the average value of these isolates(0.0821±0.0468)μg·mL^(-1)could be used as the sensitivity baseline of F.pseudograminearum to epoxiconazole.The results of variance analysis showed that there was little difference in the sensitivity of isolates from different counties to epoxiconazole with average EC_(50)ranged from 0.0508 to 0.2968μg·mL^(-1).However,the sensitivities were varied for the isolates from the same county,and the highest EC_(50)value was 210.58 times higher than that of the lowest isolates in Yucheng from Shangqiu.The results of cluster analysis showed that there was no obvious correlation between the sensitivity and the geographical origin of the isolates.There was no significant correlation between the toxicity of epoxiconazole and pyraclostrobin,fluoxonil,difenoconazole,carbendazim,phenamacril,tebuconazole.Seed dressing with epoxiconazole hindered wheat growth and inhibited root length,plant height and fresh weight.Control efficacy in greenhouse test illustrated that spraying 12.5%epoxiconazole suspension with active ingredient at 150μL·mL^(-1)had the highest control efficacy of 60.18%.In vitro test,12.5%epoxiconazole suspension not only had the highest control efficacy(79.78%)when the active ingredient at 150μL·mL^(-1),but had no effect on the growth of wheat when applied.The results of this study provided a theoretical basis for the application of epoxiconazole to control wheat stem rot and a basis for monitoring the sensitivity of the pathogen to epoxiconazole.
作者
侯颖
辛赫文
张馨
范鑫诺
刘圣明
徐建强
HOU Ying;XIN Hewen;ZHANG Xin;FAN Xinnuo;LIU Shengming;XU Jianqiang(College of Food and Bioengineering,Henan University of Science and Technology,Luoyang 471003,China;College of Horticulture and Plant Protection,Henan University of Science and Technology,Luoyang 471003,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2023年第2期307-316,共10页
Acta Phytopathologica Sinica
基金
河南省科技攻关(202102110071)
旱区作物逆境生物学国家重点实验室开放课题(CSBAAKF2021012)
河南省自然科学基金杰出青年基金(212300410015)
河南省重大科技专项(201300111600)。
关键词
河南省
假禾谷镰孢菌
氟环唑
菌丝生长速率法
敏感性
防效
Henan Province
Fusarium pseudograminearum
epoxiconazole
mycelial growth rate method
sensitivity
control efficacy
