摘要
目的探究急腹症合并重症感染患儿病原菌分布及耐药性。方法选取徐州医科大学附属沭阳医院2020年1月至2022年1月收治的100例急腹症合并重症感染患儿作为研究对象,采集患儿入院24 h内腹腔积液,依据《全国临床检验操作规程》对腹腔积液样本进行分离培养。采用全自动细菌鉴定/药敏系统监测感染病原菌种类,并参考美国临床实验室标准化协会中药敏试验标准,采用K-B纸片扩散法完成药敏试验分析。结果100例急腹症合并重症感染样本中共分离病原菌113株,包括革兰氏阳性菌25株(22.12%)、革兰氏阴性菌86株(76.11%)、真菌2株(1.78%)。大肠埃希菌、肺炎克雷伯菌、铜绿假单胞菌对庆大霉素、头孢他啶、氨苄西林、哌拉西林/他唑巴坦耐药性均>50%;对亚胺培南、氨曲南敏感性较高;不同年限大肠埃希菌、肺炎克雷伯菌、铜绿假单胞菌耐药率比较,差异均无统计学意义(P>0.05)。结论急腹症合并重症感染患儿以大肠埃希菌、肺炎克雷伯菌、铜绿假单胞菌等革兰氏阴性菌感染为主,但近2年并未表现出明显的耐药性变迁,大肠埃希菌、肺炎克雷伯菌、铜绿假单胞菌对亚胺培南、氨曲南等抗菌药仍具有较高的敏感性。
Objective To explore the distribution and drug resistance of pathogenic bacteria in children with acute abdomen complicated with severe infection.Methods A total of 100 children with acute abdomen complicated with severe infection admitted to Shuyang Hospital Affiliated to Xuzhou Medical University from January 2020 and January 2022 were selected as the research objects.The peritoneal effusion within 24 hours after admission was collected and the peritoneal effusion samples were isolated and cultured according to the national clinical examination operation procedures.The full-automatic bacterial identification/drug sensitivity system was used to monitor the species of infectious pathogens,and the K-B disk diffusion method was used to complete the drug sensitivity test analysis with reference to the Chinese medicine sensitivity test standard of the American Society for Clinical Laboratory Standards Institute.Results A total of 113 pathogenic bacteria were isolated from 100 cases of acute abdomen complicated with severe infection,including 25 Gram-positive bacteria(22.12%),86 Gram-negative bacteria(76.11%),and 2 fungi(1.78%).The resistance of Escherichia coli,Klebsiella pneumoniae,and Pseudomonas aeruginosa to Gentamicin,Ceftazidime,Ampicillin,Piperacillin/Tazobactam were all>50%.Sensitive to Imipenem and Aztreonam;there was no significant difference in the drug resistance rates of Escherichia coli,Klebsiella pneumoniae and Pseudomonas aeruginosa in different years(P>0.05).Conclusion The children with acute abdomen complicated with severe infection are mainly infected by gram-negative bacteria such as Escherichia coli,Klebsiella pneumoniae,and Pseudomonas aeruginosa,but they have not shown obvious changes in drug resistance in the past two years.Escherichia coli,Klebsiella pneumoniae,and Pseudomonas aeruginosa still have high sensitivity to Imipenem,Aztreonam and other antibiotics.
作者
任船
葛荣忠
丁辉
REN Chuan;GE Rongzhong;DING Hui(Department of General Surgery,Shuyang Hospital Affiliated to Xuzhou Medical University,Jiangsu Province,Shuyang223600,China)
出处
《中国医药导报》
CAS
2023年第10期149-151,155,共4页
China Medical Herald
基金
江苏省高层次卫生人才“六个一工程”拔尖人才项目(LGY2017077)。
关键词
急腹症合并重症感染
病原菌分布
氨基末端B型尿钠肽前体
预后
Acute abdomen complicated with severe infection
Distribution of pathogenic bacteria
N-terminal pro-B-type natriuretic peptide
Prognosis
