摘要
目的 建立落花生枝叶药材HPLC指纹图谱,运用化学计量学对不同产地落花生枝叶药材进行质量评价。方法 采用ZORBAX Eclipse XBD-C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.5%甲酸水溶液为流动相进行梯度洗脱,流速1.0 mL/min,检测波长260、310 nm,进样量10μL,柱温35℃。采用《中药色谱指纹图谱相似度评价系统》(2012版)软件进行相似度分析,结合聚类分析、主成分分析和正交偏最小二乘-判别分析(OPLS-DA),分析11个产地27批落花生枝叶药材样品的差异成分及其分布特点。结果 27批落花生枝叶药材HPLC指纹图谱相似度为0.846~0.985,标定共有峰12个,通过对照品比对指认出原儿茶酸、单咖啡酰酒石酸、咖啡酸、对香豆酸、阿魏酸和菊苣酸6个共有峰,样品一致性良好。通过聚类分析和主成分分析将样品聚为两大类,结合OPLS-DA发现峰4(单咖啡酰酒石酸)和峰11(菊苣酸)是引起样品差异性的主要标志性成分。结论 本研究建立的落花生枝叶药材HPLC指纹图谱操作简便、结果可靠,明确了不同产地落花生枝叶药材间成分的差异,可为落花生枝叶药材的资源开发和合理利用提供依据。
Objective To establish the HPLC fingerprint chromatogram of Arachis hypogaea L.stems and leaves(AHSL);To use chemometrics to evaluate the quality of AHSL from different origins.Methods Separation was performed on ZORBAX Eclipse XBD-C18 column(250 mm × 4.6 mm,5 μm);gradient elution was carried out with methanol-0.5% formic acid aqueous solution as the mobile phase;the flow rate was 1.0 mL/min;the detection wavelength was 260 nm and 310 nm;the injection volume was 10 μL;the column temperature was 35 ℃.Similarity Evaluation System of Chromatographic Fingerprint of TCM(2012 Edition),was used to conduct the similarity analysis,combined with cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis(OPLS-DA),to find the difference-related components,and to analyze their distribution characteristics among 27 batches of AHSL from 11 producing areas.Results The HPLC fingerprints of 27 batches of AHSL were established,with the similarity values between 0.846–0.985 and 12 common peaks.Six common peaks of protocatechuic acid,monocaffeoyl tartaric acid,caffeic acid,p-coumaric acid,ferulic acid and cichoric acid were identified through the comparison of reference substances.And the sample consistency was good.The samples were clustered into two categories by cluster analysis and principal component analysis.Combined with OPLS-DA,it was found that peak 4(monocaffeoyl tartaric acid) and peak 11(cichoric acid) were the main marker components that caused the differences between the different-originated samples.Conclusion The HPLC fingerprint chromatogram of AHSL established in the study is easy to operate,and the results are reliable.It has clarified the differences in components of AHSL from different producing areas,which can provide a basis for the resource development and rational utilization of the medicinal materials.
作者
李新晔
孔富康
张彤
浦益琼
LI Xinye;KONG Fukang;ZHANG Tong;PU Yiqiong(School of Pharmacy,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Experiment Center for Teaching and Learning,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处
《中国中医药信息杂志》
CAS
CSCD
2023年第4期116-121,共6页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家科技重大专项-重大新药创制(2019ZX09201004-002)。
关键词
落花生枝叶
指纹图谱
聚类分析
主成分分析
Arachis hypogaea L.stems and leaves
fingerprints
cluster analysis
principal component analysis
