摘要
植物病原真菌是农业生产的主要威胁之一,使用生物制剂防治病原真菌被认为是更安全和可持续的方式。芽胞杆菌能产生多种抗真菌活性物质(脂肽、细菌素和酶等),是目前应用最广泛的生防菌。基于芽胞杆菌及其代谢产物的生物防治剂,可有效防治植物病原真菌,在农业生产中发挥着重要的作用。本文聚焦于芽胞杆菌代谢产物的生物防治潜力及其对抗3种常见植物病原真菌(稻瘟菌、尖孢镰刀菌、灰葡萄孢菌)的拮抗属性和机理研究等,通过调研近年来已发表的芽胞杆菌代谢产物抗菌的相关文献,对几种重要的芽胞杆菌代谢产物进行介绍,并总结了芽胞杆菌代谢产物对重要植物病原真菌的抗菌效果及其机制,同时对芽胞杆菌代谢产物对病原真菌的壁膜损伤、抑制真菌孢子萌发和菌丝生长以及竞争性结合真菌DNA等机制的研究手段和效果进行了总结,期望为今后芽胞杆菌类生防制剂的制备和应用提供指导策略。
Plant pathogenic fungi are one of the major threats to agricultural production.Applying biological agents to control pathogenic fungi is widely considered to be a safer and more sustainable strategy.Bacillus species can produce a variety of antifungal active substances(lipopeptides,bacteriocins and enzymes,etc.),which is the most widely used biocontrol bacteria at present.Biocontrol agents based on Bacillus spp.and its metabolites can effectively control plant pathogenic fungi and play an important role in agricultural production.This paper focuses on the biological control potential of Bacillus metabolites and their antagonistic properties and mechanisms against three common plant pathogenic fungi(Magnaporthe oryzae,Fusarium oxysporum,and Botrytis cinerea).Several important Bacillus metabolites were introduced by investigating the related literatures published in recent years on the antifungal activities of Bacillus metabolites,and the antifungal effects and mechanisms of Bacillus metabolites on important plant pathogenic fungi were summarized.And meanwhile the research methods and effects of Bacillus metabolites on the damages of cell wall and cell membrane of pathogenic fungi,inhibition of fungal spore germination and mycelial growth,and competitive binding with fungal DNA were summarized.The aim of this review is to provide guidance for the preparation and application of Bacillus biocontrol agents in the future.
作者
王伟宸
赵进
黄薇颐
郭芯竹
李婉颖
张卓
WANG Wei-chen;ZHAO Jin;HUANG Wei-yi;GUO Xin-zhu;LI Wan-ying;ZHANG Zhuo(School of Life Sciences,Zhengzhou University,Zhengzhou 450001)
出处
《生物技术通报》
CAS
CSCD
北大核心
2023年第3期59-68,共10页
Biotechnology Bulletin
基金
河南省高等学校重点科研项目计划(20A180003)
河南省重点研发与推广专项(科技攻关)(222102110147),河南省重点研发与推广专项(科技攻关)(212102110143)。
关键词
芽胞杆菌
代谢产物
生物防治
植物病原真菌
抗真菌机制
Bacillus species
metabolite
biological control
plant pathogenic fungi
antifungal mechanism
