摘要
为明确山西省山药褐腐病病原菌的种类,从乡宁县山药种植区采集发病症状典型的山药地下块茎,采用组织分离法分离病原菌,采用菌饼接种法分析致病性,结合形态学特征和多基因联合系统发育分析对病原菌进行鉴定。结果表明,从发病地下块茎分离到5株形态特征一致的菌株,代表菌株XNSY-3在PDA培养基上菌落呈圆形,初为白色后呈淡粉色,背面中间黄色,边缘粉色,气生菌丝棉絮状;菌丝平均生长速率为1.08 cm/d;大型分生孢子呈马特形,稍弯曲,两端圆钝,2~3分隔,大小为(14.11~27.21)μm×(3.03~7.27)μm;小型分生孢子椭圆形、卵形或肾形,0~1分隔,多为无隔,大小为(7.08~17.59)μm×(3.03~6.47)μm;厚垣孢子圆形或卵圆形,可由菌丝顶端或中间产生,直径3.81~11.85μm。在以ITS联合EF-1α基因序列构建的系统发育树中,菌株XNSY-3与腐皮镰孢菌(Fusarium solani)聚于一支。致病性测定结果表明,接种XNSY-3后,发病症状与田间症状一致。结合形态学特征和分子生物学鉴定结果,确定引起山西省山药褐腐病的病原菌为腐皮镰孢菌。
In order to clarify the types of the pathogen of yam brown rot in Shanxi province,in this study,yam underground tubers with typical symptoms were collected from the yam planting area of Xiangning county,Shanxi province.The methods of tissue isolation were used to isolate the pathogen,pathogenicity was analyzed using the inoculation methods of placing the mycelial plugs on the yam tuber,the pathogen was identified with morphological characteristics and multigene phylogenetic analysis.The results showed that five strains with the same morphological characteristics were isolated from the infected underground tuber,the colony of representative strain XNSY-3 on PDA medium was round,the surface was white and then light pink,the back middle was yellow,edge was pink,and the mycelium was cotton-like.The average growth rate of mycelia was 1.08 cm/d.Macroconidium were martiella-shaped,slightly curved,and rounded and blunt at both ends,mostly with 2-3 septum,the size was(14.11-27.21)μm×(3.03-7.27)μm.Microconidium were elliptic,ovate or kidney-shaped,0-1 septum,mostly without septum,the size was(7.08-17.59)μm×(3.03-6.47)μm.Chlamydospores were round or oval,produced from the apex or middle of mycelia,the diameter was 3.81-11.85μm.In the phylogenetic tree constructed with ITS and EF-1αgene sequence,strain XNSY-3 and Fusarium solani were clustered in one branch.The results of pathogenicity test showed that the disease symptoms were consistent with those in the field after inoculation with XNSY-3.With morphological characteristics and molecular biological identification results,it was defined that the pathogen causing yam brown rot in Shanxi province was Fusarium solani.
作者
丁慧琳
翟雅鑫
薛丽芳
杨春
王建明
郝晓娟
DING Huilin;ZHAI Yaxin;XUE Lifang;YANG Chun;WANG Jianming;HAO Xiaojuan(College of Plant Protection,Shanxi Agricultural University,Taigu 030801,China;Agricultural and Rural Service Center of Yangquan Suburb,Yangquan 045000,China)
出处
《山西农业科学》
2023年第4期406-411,共6页
Journal of Shanxi Agricultural Sciences
基金
山西省基础研究计划项目(202103021224167)。
关键词
山药褐腐病
病原菌鉴定
腐皮镰孢菌
山西省
yam brown rot
pathogen identification
Fusarium solani
Shanxi province
