摘要
对干旱胁迫处理后的桑树幼苗(‘湖桑32’和‘德果1号’)进行ROS信号组织定位和叶的转录组测序,旨在从细胞和分子角度探究干旱胁迫后ROS信号在桑树组织细胞中的分布规律及与之相关的代谢通路和基因调控表达变化。结果表明:在正常情况下桑叶组织细胞中会产生少量的O^(—·)_(2),而干旱胁迫处理后桑叶组织细胞中ROS积累增多,推测ROS作为一种动态信号分子,会向毗邻细胞或远端细胞进行信号转导,进而与多种信号组分共同完成系统响应表达以调控桑树的生长发育及抗逆性。转录组数据表明:重度干旱胁迫处理后‘湖桑32’有5677个差异表达基因显著多于‘德果1号’(5575),且差异显著基因(DEGs)主要富集在半乳糖代谢、淀粉和蔗糖代谢、果糖和甘露糖代谢、碳代谢等通路中,推测‘湖桑32’的抗旱性与渗透调节物质的合成有关。同时,挑选11个基因进行qRT-PCR验证,其中11个DEGs的表达趋势与对应的RNA-Seq数据一致。研究也发现调控MKK 4/5和WRKY22表达的相关基因LOC 21384958和LOC21396104,在弱抗旱桑‘德果1号’中分别上调表达0.72(P<0.05)和2.16(P<0.05),而相同的基因在强抗旱桑‘湖桑32’中未显著表达,推测‘德果1号’叶片枯黄或死亡的原因可能与ROS信号参与MKK4/5、MPK3/6和WRKY22级联途径有关。
To explore the distribution of ROS signal in mulberry tissue cells and related metabolic pathways and gene regulation expression changes after drought stress from a cellular and molecular perspective,the tissue localization of ROS signal and the transcriptome of leaves of mulberry seedlings(‘Husang 32’and‘Deguo 1’)after drought stress were investigated.The study would provide new insights into the drought resistance mechanism of mulberry.The results showed that a small amount of O^(—·)_(2) was produced in mulberry leaf tissue cells under normal conditions,but the accumulation of ROS in mulberry leaf tissue cells increased after drought stress treatment.It was suggested that ROS,as a dynamic signal molecule,conduct signal transduction to adjacent cells or distant cells,and then complete the system response expression together with various signal components to regulate the growth and development and stress resistance of mulberry.Transcriptome data showed that 5677 differentially expressed genes in‘Husang 32’were significantly more than‘Deguo 1’(5575)after severe drought stress,and DEGs were mainly enriched in galactose metabolism,starch and sucrose metabolism,fructose and in the pathways of mannose metabolism and carbon metabolism.It was speculated that the drought resistance of‘Husang 32’was related to the synthesis of osmotic regulators.Eleven genes were selected for qRT-PCR validation,and the expression trends of 11 DEGs were consistent with the corresponding RNA-Seq data.The study also found that the related genes LOC 21384958 and LOC21396104,which regulating the expression of MKK4/5 and WRKY22,were up-regulated by 0.72(P<0.05)and 2.16(P<0.05)respectively in the weak drought-resistant mulberry‘Deguo 1’,while the same genes were not significantly expressed in the strong drought-resistant mulberry‘Husang 32’.It was thought that the cause of‘Deguo 1’leaf yellowing or death might be related to the involvement of ROS signaling in the MKK4/5,MPK3/6 and WRKY22 cascade pathways.
作者
祁伟亮
任迎虹
杨财容
乔岩
曾睿
黄仁维
乔义林
QI Weiliang;REN Yinghong;YANG Cairong;QIAO Yan;ZENG Rui;HUANG Renwei;QIAO Yilin(College of Agriculture and Forestry,Longdong University,Qingyang,Gansu 745000,China;Collaborative Innovation Center for Longdong Dryland Crop Germplasm Improvement and Industrialization,Qingyang,Gansu 745000,China;College of Chemistry and Life Science,Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources,Chengdu Normal University,Chengdu,Sichuan 611130,China)
出处
《干旱地区农业研究》
CSCD
北大核心
2023年第2期50-60,共11页
Agricultural Research in the Arid Areas
基金
陇东学院博士基金(901110050311)
甘肃省高等学校创新基金(2022B-210)
庆阳市创新基地和人才计划项目(QY2021B-F003)
甘肃省高等学校创新创业教育示范课项目(序号4)
教育部产学合作协同育人项目(202102022080)
陇东学院创新创业项目(20221136,20221144)
国家自然科学基金(31960441)。
关键词
桑树
干旱胁迫
活性氧
信号传导
转录组分析
mulberry
drought stress
reactive oxygen species
signal transduction
transcriptome analysis
