摘要
以食物和水作为传播基质的诺如病毒(NoV)能够在全球范围内引发急性肠胃炎,但目前没有特效的抗病毒药物,只能通过快速检测在早期筛查识别NoV,从而预防NoV的传播并及时做出干预措施。传统的检测方法耗时耗力,操作繁琐,不利于NoV的即时检测,因此NoV快速检测技术的建立成为研究者不断探究的问题。目前已有一些快速检测方法的报道,但由于NoV体外不可培养的特性,很难进行方法间的试验比较。因此,本文针对目前报道较多的快速检测技术进行了梳理,综述了分子生物学、免疫学、生物传感器等技术在NoV快速检测中的应用及发展趋势,从方法的检出限、检测时间以及优缺点等进行了整理比较,以期为将来NoV的现场快速检测及后续相关新型检测技术的研究提供参考。
Norovirus(NoV),which transmits through food and water,can cause acute gastroenteritis worldwide.However,there is no specific antiviral drug.To prevent the spread of NoV outbreaks and intervene in time,only rapid detection and screening can identify NoV at the early stage.Traditional detection methods are time-consuming,laborintensive,and the operation is cumbersome,which is not suitable for the real-time detection of NoV.Therefore,the establishment of NoV rapid detection technology has become an ongoing issue for researchers.Though many rapid detection methods have been reported,it is difficult to compare these methods because of the unculturable nature of NoV in vitro.Therefore,the currently reported rapid detection technologies are sorted out,and the research status at home and abroad is briefly introduced.The application and development trend of molecular biology,immunology,biosensor and other technologies in the rapid detection of NoV is reviewed.The detection limit,detection time,advantages and disadvantages of the methods are compared.It is expected to provide a reference for the on-site rapid detection of NoV and subsequent research of relevant novel detection technologies.
作者
杨艳歌
吴占文
李涛
李红娜
王帅
孙冬梅
袁飞
YANG Yange;WU Zhanwen;LI Tao;LI Hongna;WANG Shuai;SUN Dongmei;YUAN Fei(Key Laboratory of Food Quality and Safety for State Market Regulation,Chinese Academy of Inspection and Quarantine,Beijing 100176,China;College of Life Science and Technology,Heilongjiang Bayi Agricultural Reclamation University,Heilongjiang Daqing 163000,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2023年第1期131-136,共6页
Chinese Journal of Food Hygiene
基金
中国检验检疫科学研究院基本科研业务费专项(2022JK36)
国家重点研发计划(2022YFF0607900)。
