摘要
分别采用不同分子量的腐殖质(富里酸、胡敏酸、胡敏素)培育独山矿区锑污染土壤,以独山锑矿区指示性植物头花蓼为受试植物进行盆栽实验,采用ICP-MS测定头花蓼体内重金属(Sb、Cu、Cd、Pb、As)的积累状况,用两步连续提取方法测定了头花蓼体内Sb的赋存形态,并通过测定头花蓼植株的叶片叶绿素含量、抗氧化酶活性,研究了腐殖质对Sb污染土壤下头花蓼植株生理生化特性的影响。结果表明:腐殖质添加后使得头花蓼叶绿素总量整体呈现上升的趋势,加强了头花蓼光合作用;头花蓼的抗氧化酶活性表现为在低添加量下,胡敏素实验组与胡敏酸实验组中丙二醛含量下降,SOD酶活性升高,CAT酶活性下降;说明胡敏素和胡敏酸在低添加量下能够有效缓解头花蓼所受到的Sb毒害,而高添加量富里酸对头花蓼有一定损害;低添加量的胡敏酸能够不同程度缓解头花蓼植株所受到的重金属Sb、As、Cd、Cu胁迫;相比于胡敏素和胡敏酸,富里酸更容易活化头花蓼体内的Sb,在4%富里酸添加量下,头花蓼体内乙醇提取态的Sb含量从16%上升到30%;盐酸态Sb含量由10%上升到16%,说明富里酸能大幅度促进头花蓼体内残渣态Sb向更活跃的乙醇提取态与盐酸提取态转化;胡敏酸对头花蓼中的Sb赋存形态表现出了低浓度活化而高浓度钝化的现象;胡敏素则能够有效钝化头花蓼体内的Sb,相比于空白对照组,0.5%的胡敏素添加后头花蓼体内残渣态Sb含量由73%迅速增加到83%。
Antimony contaminated soil in Dushan mining area was cultivated by different molecular weights humus(fulvic acid, humin acid and humin),and the indicative plant cephalopodium in Dushan antimony mining area was used as the subject plant for potting experiments.The accumulation of heavy metals(Sb, Cu, Cd, Pb, As) in the cephalus was determined by ICP-MS,and the Sb occurrence form in the cephalus was determined by a two-step continuous extraction method.The humus pair Sb was studied by measuring the leaf chlorophyll content and antioxidant enzyme activity in the cephalus plant, and based on this, effects of humus on the physiological and biochemical characteristics of plants under Sb contaminated soil were studied.The results showed that the total amount of chlorophyll in the head flower was increased after the addition of humus, as a result, the photosynthesis of the head flower was strengthened.The antioxidant enzyme activity of cephalopodium were manifested as follows: under the low addition amount of humus, the malondialdehyde content decreased both in humin group and humic acid group.Meanwhile, SOD enzyme activity increased, and CAT enzyme activity decreased.The results above showed that humin and humic acid could effectively alleviate the Sb poisoning of cephalopodium under low addition amount.While the high amount of fulvic acid had some damage to the head flower.The low added amount of humic acid could alleviate the heavy metal(such as Sb, As, Cd and Cu) stresses of the head flower plant to varying degrees.In addition, compared with humin and humic acid, fulvic acid was easier to activate Sb in the body of cephalus.The content of Sb in the ethanol extracted state of cephalus was increased from 16% to 30% and the content of Sb hydrochloride increased from 10% to 16% under the addition of 4% fulvic acid.This indicated that fulvic acid could greatly promote the conversion of Sb from residual state in the body of cephalus to the more active ethanol extraction state and the hydrochloric acid extraction state.The Sb-endowment morphology of humic acid on head flowers exhibited the phenomenon of low concentration activation and high concentration passivation.Humin was able to effectively passivate the Sb in the body of the head flower.Compared with the blank control group, the residual Sb content of 0.5% of the headflower after the addition of humin was rapidly increased from 73% to 83%.
作者
冯于航
杨爱江
胡霞
宋霞
贺赟
FENG Yuhang;YANG Aijiang;HU Xia;SONG Xia;HE Yun(School of Resource and Environmental Engineering,Guizhou University,Guiyang,Guizhou 550025,China;Field Scientific Observation and Research Station of the Ministry of Education of Guizhou Karst Environmental Ecosystem,Guiyang,Guizhou 550025,China)
出处
《中国无机分析化学》
CAS
北大核心
2023年第4期335-348,共14页
Chinese Journal of Inorganic Analytical Chemistry
基金
贵州省科技计划项目(黔科合基础[2020]1Y195)
贵州省教育厅省级重点学科建设项目(黔学位合字ZDXK[2016]11)
贵州省国内生态学一流学科建设项目(GNYL[2017]007)。
关键词
锑
头花蓼
腐殖质
生化特性
Sb
polygonum cuspidatum
humus
biochemical properties
