摘要
凝集素类受体激酶(lectin receptor-like kinase, LecRLKs)是一类在植物应答多种生物/非生物胁迫中发挥重要作用的类受体激酶。本研究在小麦与条锈菌互作的转录组中筛选到1个在小麦与条锈菌非亲和互作中显著上调表达的LecRLKs基因TaLecRLK1。该基因全长2 292 bp,编码蛋白含有1个胞外信号肽、B-lectin结构域、PAN_AP结构域、跨膜域和胞内酪氨酸激酶域。qRT-PCR分析表明:TaLecRLK1在小麦与条锈菌非亲和互作早期诱导表达,在烟草及小麦原生质体中瞬时表达,TaLecRLK1-GFP定位在细胞膜上。利用大麦条纹花叶病毒介导的基因沉默技术(BSMV-VIGS)沉默TaLecRLK1,接种无毒性条锈菌小种CYR23后,沉默叶片表面产生少量夏孢子堆,组织学观察发现沉默植株中条锈菌菌丝长度增长、侵染点附近活性氧积累面积减少;TaPR1、TaPR2、TaPR5的表达受到抑制,TaCAT和TaSOD则被迅速诱导表达。综上所述,TaLecRLK1对小麦抗条锈病起到正调控作用。
Lectin receptor-like kinase(LecRLK) is an important class of receptor-like protein kinases in plants that plays vital roles in plant response to various biotic and abiotic stresses. In this study, by analyzing the transcriptome data of wheat plants infected by Puccinia striiformis f. sp. tritici(Pst), we obtained a wheat lectin-like receptor kinase gene TaLecRLK1 that was significantly up-regulated during the wheat-Pst incompatible interaction. TaLecRLK1 contains a N-terminal signal peptide, an extracellular B-lectin domain, a PAN_AP domain, a transmembrane domain and an intracellular Pkinase-Tyr domain. Quantitative real-time PCR verified that TaLecRLK1 expression was induced in the early stage of wheat resistance to avirulent Pst infection. TaLecRLK1-GFP was located on the cell cytoplasmic membrane of Nicotiana benthamiana and wheat protoplasts. Virus induced gene silencing(VIGS) of TaLecRLK1 by barley stripe mosaic virus(BSMV) weakened wheat resistance to stripe rust, leading to sporadic production of uredinospores on TaLecRLK1-silencing wheat leaves challenged with Pst CYR23 that is avirulent on the control plants. Histological observation revealed longer infection hyphae in TaLecRLK1-silencing plants, along with decreased reactive oxygen species(ROS) accumulation per infection site compared with that in the control plants. In addition, the expression of defense-related genes TaPR1, TaPR2 and TaPR5 were decreased in TaLecRLK1-silencing plants, while the expression of TaCAT and TaSOD responsible for ROS scavenge were rapidly induced. These results indicate that TaLecRLK1 functions as positive regulator of wheat resistance to Pst.
作者
王建锋
甘鹏飞
徐静华
汤春蕾
康振生
王晓杰
WANG Jianfeng;GAN Pengfei;XU Jinghua;TANG Chunlei;KANG Zhensheng;WANG Xiaojie(State Key Laboratory of Crop Stress Biology for Arid Areas,College of Plant Protection,Northwest A&F University,Yangling 712100,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2023年第1期61-71,共11页
Acta Phytopathologica Sinica
基金
国家重点研发计划(2021YFD1401000)
国家小麦产业技术体系(CAR-03)
中央高校基本科研业务费专项资金资助(2452020223)。
