摘要
利用单个飞秒振荡器为激发光源搭建了光谱聚焦相干反斯托克斯拉曼散射(CARS)显微成像系统,使用二向色镜获得了泵浦光与斯托克斯光,开展了聚苯乙烯样品低指纹区的光谱聚焦CARS二维与三维显微成像研究。在光谱聚焦CARS二维成像研究中,通过改变泵浦-斯托克斯的延迟时间,获得了随延迟时间变化的光谱聚焦CARS信号强度谱,通过与聚苯乙烯拉曼光谱的对比,分析了在改变泵浦-斯托克斯的延迟时间过程中被激发的拉曼振动的切换情况。同时,在固定的拉曼振动模式,通过对聚苯乙烯样品进行断层扫描,实现了对聚苯乙烯样品的三维CARS成像。
A spectral focusing coherent anti-Stokes Raman scattering(CARS)microimaging system was developed using a femtosecond oscillator as the excitation source.Pump light and Stokes light were obtained by using dichromatic mirrors.Two-and three-dimensional microscopic imaging studies of the spectral focusing CARS imaging in the low-fingerprint region of polystyrene samples were carried out.While changing the delay time of pump-Stokes during two-dimensional imaging,CARS signal intensity spectra that varied with delay time were obtained.The switching of excited Raman vibrations in the process of changing the pump-Stokes delay time was analyzed by comparing it to the polystyrene Raman spectra.Furthermore,the three-dimensional CARS imaging was realized by tomography in the fixed Raman vibration mode for polystyrene samples.
作者
张宁
侯国忠
邓岩岩
刘硕
王雨雷
吕志伟
夏元钦
Zhang Ning;Hou Guozhong;Deng Yanyan;Liu Shuo;Wang Yulei;Lü Zhiwei;Xia Yuanqin(Center for Advanced Laser Technology,School of Electronic and Information Engineering,Hebei University of Technology,Tianjin 300401,China;Hebei Key Laboratory of Advanced Laser Technology and Equipment,Tianjin 300401,China;State Key Laboratory of Tunable Laser Technology,Harbin Institute of Technology,Harbin 150080,Heilongjiang,China)
出处
《激光与光电子学进展》
CSCD
北大核心
2022年第24期217-223,共7页
Laser & Optoelectronics Progress
基金
国家自然科学基金(61675057,62075056)
河北省自然科学基金(F2021202055)。
