摘要
TCP(Teosinte branched1/Cycloidea/Proliferating cell factor)转录因子是植物特有的转录因子,能够结合特定的启动子序列来调节其靶基因的表达水平.TCP为植物中保守和广泛存在的转录因子家族,不同植物进化出多种方式精准调控其下游相关因子,并且自身基因表达也被严格地调控.TCP不仅可以在植物根、茎、叶、花、果实和维管组织发育中起到重要调控作用,还可以参与植物次生代谢、生物胁迫和非生物胁迫等重要生理学过程.近年来,有关TCP对植物生长发育调控机制的研究越来越多,尤其是对上游调控因子及下游靶基因生物学功能的相关研究.本文综述了TCP转录因子在维管组织发育、生命周期转变、植物逆境胁迫等过程中的调控机制,以及miRNA-TCP、TCP与染色质重塑等相互作用调控网络的最新进展.最后,对目前TCP研究中存在的问题和未来的研究方向进行了讨论和展望.
Transcription factors(TFs) are a group of protein molecules that can bind to a specific gene with a specific sequence specificity, thus ensuring that the target gene is expressed at a specific time and in space at a specific intensity. The plantspecific TFs of the TCP family(for Teosinte branched 1 in corn, CYCLOIDEA(CYC) in snapdragon(Antirrhinum majus)and PROLIFERATING CELL FACTOR 1/2 in rice) are characterized by an N-terminal non-canonical beta helix-loophelix(b HLH) domain known as the TCP domain, and regulate the spatiotemporal expression of genes by binding to specific promoter sequences. TCP TFs play important roles in regulating the development of roots, stems, leaves, flowers,fruits, and vascular tissues, and participate in physiological processes such as plant secondary metabolism, plant immunity,and the responses to biotic and abiotic stress. The TCP TF family is conserved and widespread in plants. Based on the TCP domain sequence, TCP TFs are categorized into Class I and Class II;based on the amino acid sequence of the basic region and b HLH domain, the Class II TCP TFs can be further divided into CINCINNATA(CIN)-like TCPs(CIN) and CYC-like TCPs(CYC-C).In recent years, increasing numbers of studies have shed light on how TCP TFs regulate plant growth and development,especially on the functions of upstream regulators and downstream target genes. Plants have evolved multiple ways to accurately regulate their downstream related factors, and their own gene expression is also strictly regulated. TCP genes have also been analyzed in various plants and found to take part in the regulation of circadian rhythms and the biosynthesis and signaling pathways of plant hormones, among other functions. Other studies have investigated the regulatory relationships between TCP TFs and micro RNAs. Additionally, members of the TCP family are often functionally redundant, making it difficult to study their functions;therefore, gene-editing technology and high-throughput sequencing have opened up new avenues to study this TF family. Here, we summarize recent advances in our understanding of how TCP activity is translated to the dynamic spatiotemporal control of cell-fate determination, life-cycle transitions, dormancy release, seed germination, leaf development, outgrowth of shoot branches, growth-repressing micro RNAs, and interactions with the chromatin remodeling machinery to modulate phytohormone responses. Furthermore, we discuss existing problems and future directions in TCP research, in particular the role of TCP-related protein modification in plant growth and development and stress responses. Finally, we provide perspectives on the future research that is needed to gain a deep understanding of the diverse regulatory networks of TCP TFs in plant growth and development.
作者
唐羽翔
高旭
崔亚宁
许会敏
于静娟
Yuxiang Tang;Xu Gao;Yaning Cui;Huimin Xu;Jingjuan Yu(College of Biological Sciences,China Agricultural University,Beijing 100193,China;National Demonstration Center for Experimental Life Science Education,China Agricultural University,Beijing 100193,China;College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,China)
出处
《科学通报》
EI
CAS
CSCD
北大核心
2022年第33期3964-3975,共12页
Chinese Science Bulletin
基金
国家自然科学基金(31800504,32000483)
中国农业大学生命科学实验教学中心能力提高项目和研究生教改项目(JG202209)资助。
关键词
TCP转录因子
生物学功能
逆境胁迫
调控网络
TCP transcription factor
biological function
adversity stress
regulatory network
