摘要
以小球藻藻种为原料,通过实验室培育得到藻粉,再通过碱性蛋白酶制备小球藻抗氧化肽,以DPPH自由基清除率为指标,通过单因素和响应面设计探究加酶量、pH、酶解时间和反应温度对抗氧化肽的抗氧化性的影响,通过超滤离心对多肽进行分离,测定了各组分的DPPH自由基清除率,再通过氨基酸分析仪和凝胶渗透色谱仪测定了DPPH自由基清除率最好的多肽组分的氨基酸组成和分子量,又测定了它的热稳定性和贮藏稳定性。结果表明:小球藻蛋白制备抗氧化肽的最佳条件为:pH为7,反应温度为40℃,反应时间为30 min,加酶量为4000 U·g^(-1),此时DPPH自由基的清除率是58.03%。超滤分离得到五个组分,其中分子质量为5 KD-10 KD的DPPH自由基清除率最佳,多肽中的抗氧化氨基酸含量为49.9%,它的相对分子量为474,抗氧化肽有着良好的热稳定性以及贮藏稳定性。
Chlorella were used as raw materials to obtain algae powder through laboratory cultivation,and then chlorella antioxidant peptides were prepared by alkaline protease.With DPPH free radical scavenging rate as the index,the effects of enzyme dosage,pH,enzymolysis time and reaction temperature on antioxidant peptides were investigated by single factor and response surface design.The peptides were separated by ultrafiltration and centrifugation,and the DPPH free radical scavenging rate of each component was determined.The amino acid composition and molecular weight of the polypeptide components with the best DPPH free radical scavenging rate were determined by amino acid analyzer and gel permeation chromatograph,and the results were also determined.The results showed that the optimum conditions for preparing antioxidant peptides from chlorella protein were as follows:pH 7,reaction temperature 40℃,reaction time 30 min,enzyme dosage 4000 U·g^(-1),and the scavenging rate of DPPH free radical was 58.03%.Five components were separated by ultrafiltration,among which DPPH with molecular weight of 5 KD-10 KD had the best free radical scavenging rate.The antioxidant amino acid content in the polypeptide was 49.9%,and its relative molecular weight was 474.The antioxidant peptide had good thermal stability and storage stability.
作者
宋林
张炜
乜世成
高红
沈艳洁
隋成博
荆永康
SONG Lin;ZHANG Wei;NIE Shi-cheng;GAO Hong;SHEN Yan-jie;SUI Cheng-bo;JING Yong-kang(School of Chemical,Qinghai Normal University,Xining 810008,China)
出处
《化学研究与应用》
CAS
北大核心
2023年第1期135-144,共10页
Chemical Research and Application
基金
青海省科学技术厅基金项目(2021-SF-139)资助。
关键词
小球藻
抗氧化肽
DPPH自由基
响应面优化
超滤离心
chlorella
antioxidant peptides
DPPH free radicals
response surface optimization
ultrafiltration centrifugal
