摘要
背景:纳米羟基磷灰石是优良的生物活性材料,对脱矿牙釉质和牙本质具有一定的保护作用,但其溶液对脱矿牙本质再矿化的作用及其机制尚不明确。目的:探讨纳米羟基磷灰石用于防治牙本质敏感症和牙本质龋的可行性。方法:收集废弃的人双尖牙标本,制备出72份牙本质薄片,分6组处理:A组脱矿后浸入10%纳米羟基磷灰石溶液中,B组脱矿后浸入6%生物活性玻璃溶液中,C组脱矿后浸入10%纳米羟基磷灰石与6%生物活性玻璃复合溶液中,D组脱矿后浸入0.05%NaF溶液中,E组脱矿后浸入人工唾液中,F组脱矿后浸入去离子水中,每组12个样本。处理14 d后,扫描电镜下观察样本工作面,利用能谱分析仪检测样本表面成分,激光共聚焦显微镜下检测牙本质罗丹明B染色情况。结果与结论:①扫描电镜观察:A组牙本质小管口被完全封闭,牙本质表面被一层紧密连接的颗粒物均匀覆盖;C组牙本质小管部分封闭;B、D、E组牙本质表面及牙本质小管内见少量散在颗粒物,牙本质小管口呈开放状;F组牙本质表面及牙本质小管内未见颗粒物沉积,牙本质小管口呈开放状态;②X射线能谱分析:A组Ca/P比为1.61,最接近健康牙本质理论值,Ca/P比高于B、E、F组(P<0.05),与C组Ca/P比无差异(P>0.05);③激光共聚焦显微镜检测:A组荧光强度和荧光面积最小,平均荧光强度小于其他5组(P<0.05);C组平均荧光强度小于B、D、E、F组(P<0.05);④结果表明:纳米羟基磷灰石对脱矿牙本质具有良好的再矿化作用,纳米羟基磷灰石与生物活性玻璃联合应用亦能沉积在牙本质表面,但并无增效作用,封闭效果不及单独使用纳米羟基磷灰石。
BACKGROUND:Nano hydroxyapatite is an excellent bioactive material,which has a certain protective effect on demineralized enamel and dentin,but the effect and mechanism of its solution on remineralization of demineralized dentin are not clear.OBJECTIVE:To explore the feasibility of nano hydroxyapatite in the prevention and treatment of dentin sensitivity and dentin caries.METHODS:Abandoned human bicuspid specimens were collected,and 72 dentin slice samples were prepared from isolated bicuspids.The samples were randomly divided into 6 groups,with 12 samples per group:After demineralization,each group was immersed in 10%nano hydroxyapatite solution(group A),6%bioactive glass solution(group B),10%nano hydroxyapatite and 6%bioactive glass composite solution(group C),0.05%sodium fluoride(group D),artificial saliva(group E),and deionized water(group F).After 14 days of treatment,working surfaces of samples were observed by scanning electron microscope.The surface composition was detected with energy spectrum analyzer.Dentin Rhodamine B staining was detected under a confocal laser microscope.RESULTS AND CONCLUSION:(1)Scanning electron microscopy:The dentinal tubules orifice in group A was completely sealed,and the surface of the dentin was uniformly covered by a layer of tightly connected granular material.The dentinal tubules in group C were partially sealed.A small amount of scattered particles were found on the dentin surface and in the dentinal tubules of group B,group D and group E,and the dentinal tubules were open.In group F,there was no particulate matter deposition on the dentin surface or in the dentinal tubules,and the dentinal tubules were open.(2)X-ray energy dispersive spectrocopy results:The Ca/P ratio of group A was 1.61,which was closest to the theoretical value of healthy dentin;the Ca/P ratio was higher in group A than that in the groups B,E,and F(P<0.05).There was no significant difference in Ca/P ratio between group A and group C(P>0.05).(3)Laser confocal microscopy test results showed that the fluorescence intensity and fluorescence area of group A were the smallest,and the mean fluorescence intensity was smaller in group A than that in other five groups(P<0.05).The mean fluorescence intensity of group C was lower than that of groups B,D,E and F(P<0.05).(4)It is indicated that nano hydroxyapatite has a good remineralization effect on demineralized dentin.The combination of nano hydroxyapatite and bioactive glass can also deposit on the dentin surface,but there is no synergistic effect.The sealing effect is not as good as that of nano hydroxyapatite alone.
作者
于蓝宁
王倩
靳友士
费晓文
王青山
Yu Lanning;Wang Qian;Jin Youshi;Fei Xiaowen;Wang Qingshan(Binzhou Medical University Hospital,Binzhou 256603,Shandong Province,China;Shangdi Branch of Ruitai Stomatological Hospital,Beijing100085,China)
出处
《中国组织工程研究》
CAS
北大核心
2023年第25期3964-3970,共7页
Chinese Journal of Tissue Engineering Research
基金
山东省自然科学基金项目(ZR2018LH010),项目负责人:王青山。
