摘要
目的研究β2-微球蛋白(β2-microglobulin,β2M)、血小板膜糖蛋白CD62P、CD63表达水平对原发性免疫性血小板减少症(primary immune thrombocytopenia,ITP)的临床诊断价值。方法选取我院接受治疗的45例ITP患者作为ITP组,同期选取40例中重度贫血患者作为贫血症组、40例健康体检者作为对照组。比较三组的基线资料,检测三组血小板(platelet,PLT)、血小板平均体积(mean platelet volume,MPV)、血小板比容(platelet volume,PCT)、血小板分布宽度(platelet distribution width,PDW),采用酶联免疫吸附法检测β2M、CD62P、CD63表达水平,并进行比较;分析ITP的影响因素,ROC曲线分析β2M、CD62P、CD63表达水平对ITP的诊断价值。结果对照组、贫血症组、ITP组生命体征、PLT、MPV、PCT、PDW等指标比较差异有统计学意义(P<0.05),其中ITP组患者生命体征、PLT、MPV、PCT、PDW等指标水平最低,其次是贫血症组,对照组生命体征、PLT、MPV、PCT、PDW等指标水平最高(P<0.05)。对照组、贫血症组、ITP组血清中β2M、CD62P、CD63表达水平依次升高(P<0.05)。Logistic回归分析结果显示,血清β2M、CD62P、CD63水平升高是ITP的影响因素。ROC曲线分析显示,与β2M、CD62P、CD63单项诊断相比,联合诊断对ITP诊断价值较高(P<0.05)。结论β2M、CD62P、CD63参与ITP的发生与发展,在ITP的早期诊断中,三项联合诊断特异度和敏感度较高。
Objective To study the clinical diagnostic value of expression levels ofβ2-microglobulin(β2M),platelet membrane glycoproteins CD62P and CD63 in primary immune thrombocytopenia(ITP).Methods In total,45 patients with ITP who were treated in our hospital were selected as ITP group,40 patients with moderate or severe anemia as anemia group and 40 healthy people undergoing physical examination during the same period as the control group.The baseline data of the three groups were compared,and platelet(PLT),the mean platelet volume(MPV),plateletcrit(PCT)and platelet distribution width(PDW)of the three groups were detected.The expression levels ofβ2M,CD62P and CD63 were detected by enzyme-linked immunosorbent assay and compared.The influencing factors of ITP were analyzed,and the diagnostic value of expression levels ofβ2M,CD62P and CD63 in ITP was analyzed by ROC curve.Results There were statistical differences among the control group,anemia group and ITP group with respect to vital signs,PLT,MPV,PCT,and PDW(P<0.05),among which the ITP group had the lowest level of vital signs,PLT,MPV,PCT,and PDW,followed by anemia group and the control group(P<0.05).The serum expression levels ofβ2M,CD62P and CD63 was the lowest in control group,followed by anemia group and ITP group(P<0.05).Logistic regression analysis showed that the elevated levels of serumβ2M,CD62P and CD63 were the influencing factors of primary ITP.Receiver operating characteristic(ROC)curve analysis showed that the combined detection had a higher diagnostic value for ITP than the detection ofβ2M,CD62P and CD63 alone(P<0.05).Conclusionβ2M,CD62P and CD63 are involved in the occurrence and development of ITP.The specificity and sensitivity of combined detection of the three are relatively higher in the early diagnosis of ITP.
作者
徐锋
XU Feng(Clinical Laboratory Center,Central Hospital of Enshi Tujia and Miao Autonomous Prefecture,Hubei Province,Enshi 445000,China)
出处
《河北医科大学学报》
CAS
2022年第11期1323-1327,共5页
Journal of Hebei Medical University
基金
恩施市卫生和计划生育委员会科研项目(WX19C25)。
