摘要
为挖掘香芋软腐病的生物防治资源,探讨生物炭环境协同解淀粉芽孢杆菌(Bacillus amyloliquefaciens)对香芋软腐病病原菌(Dickeya fangzhongdai)的拮抗作用以及生物防治效果,利用平板对峙法从健康香芋植株中筛选出一株拮抗Dickeya fangzhongdai ZXC1的菌株P4并对其进行分类鉴定;采用竞争性培养的方法验证生防菌的拮抗效果;利用对峙平板法,根据抑菌圈大小明确生物炭是否能够促进生防菌分泌拮抗物质;利用计数统计法测定不同浓度的生物炭环境中致病菌及拮抗菌的数目变化;不同含量生物炭环境下进行盆栽试验,分析生物炭环境与生防菌作用下香芋软腐病病原菌种群数量变化,并统计香芋的发病情况。结果表明,P4菌株对香芋软腐病病原菌具有良好的抑制作用;适量生物炭能够加强生防菌对香芋软腐病的防治效果,并且能直接抑制病原菌致病;生物炭直接刺激生防菌增强其分泌拮抗物质的效果很微弱;生防菌与病原菌的数目随生物炭浓度的上升而增加。P4菌株可作为香芋软腐病的生防菌。加入适量的生物炭能够促进土壤中生防菌生长繁殖并使其占据良好的生态位,加强生防菌对香芋软腐病的生防效果,并且仅加入生物炭也能够抑制病原菌致病。
In order to explore the biological control resources of taro soft rot,the antagonistic effect of biochar environment and Bacil⁃lus amyloliquefaciens on the pathogen of taro soft rot(Dickeya fangzhongdai)and the biological control effect were discussed.A strain P4 antagonistic to Dickeya fangzhongdai ZXC1 was screened from healthy taro plants by plate confrontation method and classified and identified.The antagonistic effect of biocontrol bacteria was verified by competitive culture.Using the confrontation plate method,whether biochar could promote the secretion of antagonistic substances by biocontrol bacteria was determined according to the size of inhibition zone;the counting statistics method was used to determine the number of pathogenic bacteria and antagonistic bacteria in different concentrations of biochar environment.Pot experiments were carried out under different biochar contents to analyze the chang⁃es in the number of pathogenic bacteria of Dickeya fangzhongdai under biochar environment and biocontrol bacteria,and the incidence of taro was counted.The results showed that P4 strain had a good inhibitory effect on the pathogen of Dickeya fangzhongdai.Appropri⁃ate amount of biochar could enhance the control effect of biocontrol bacteria on soft rot of taro,and could directly inhibit the pathoge⁃nicity of pathogenic bacteria;the effect of biochar directly stimulating biocontrol bacteria to enhance their secretion of antagonistic sub⁃stances was very weak.The number of biocontrol bacteria and pathogens increased with the increase of biochar concentration.P4 strain could be used as biocontrol bacteria of taro soft rot.Adding an appropriate amount of biochar could promote the growth and reproduc⁃tion of biocontrol bacteria in soil to occupy a good ecological niche,enhance the biocontrol effect of biocontrol bacteria on taro soft rot,and only adding biochar could also inhibit the pathogenic bacteria.
作者
王莹乐
李泳霖
梁雪
黄淑芬
张炼辉
廖立胜
周筱帆
WANG Ying-le;LI Yong-lin;LIANG Xue;HUANG Shu-fen;ZHANG Lian-hui;LIAO Li-sheng;ZHOU Xiao-fan(Integrative Microbiology Research Centre/Guangdong Province Key Laboratory of Microbial Signals and Disease Control,South China Agricultural University,Guangzhou 510642,China)
出处
《湖北农业科学》
2022年第21期79-85,共7页
Hubei Agricultural Sciences
基金
热带亚热带农业微生物种质资源研究与选育项目(2018B020205003)
韶关市科技计划项目(200805094530618)。
