摘要
目的:研究经辐照处理的红景天提取物(RRE)中活性成分的变化及其降血糖作用。方法:分别用5,10,20,30 kGy辐照剂量的Co-γ射线对红景天进行辐照处理。通过高效液相色谱法(HPLC)对辐照前(0 kGy)后RRE化合物进行分析,对用链脲佐菌素(STZ)腹腔注射建立的1型糖尿病小鼠模型进行灌胃,定期测定小鼠体重、空腹血糖值、摄食量和饮水量,最后1 d测定口服葡萄糖耐量,解剖后测定脏器系数、血清总胆固醇(TC)、糖化血红蛋白(GHb)、总甘油三酯(TG)、丙二醛(MDA)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的含量。结果:辐照后的RRE中红景天苷的含量均显著减少(P<0.05),在5,10,20 kGy时络塞维的含量显著增加(P<0.05),在20 kGy时检测到酪醇。与模型组相比,灌胃辐照处理的RRE后小鼠体重有所增长,摄食量、饮水量和血糖水平降低而食物利用率显著提高(P<0.05),TC、GHb、TG和MDA水平显著降低(P<0.05),而SOD和CAT活力显著提高(P<0.05),脏器肿大的现象得到改善,在20 kGy时效果较好,且与其他辐照剂量存在显著性差异(P<0.05)。结论:辐照处理后RRE中化合物组成及含量产生变化,辐照前后RRE均能够降低1型糖尿病小鼠的糖尿病水平,且在20 kGy的辐照剂量时效果较好。
Objective:This study aimed to study the changes of active components in irradiated Rhodiola rosea extracts(RRE)and their hypoglycemic effect.Methods:Rhodiola rosea was irradiated withCo-γrays at doses of 5,10,20 and 30 kGy,respectively.High performance liquid chromatography(HPLC)was used to analyze RRE compounds before(0 kGy)and after irradiation.The type 1 diabetic mouse models established by intraperitoneal injection of Streptozotocin(STZ)were given a gavage.The body weight,fasting blood glucose,food intake and water intake of mice were measured regularly.Oral glucose tolerance was measured on the last day,after dissection,organ coefficient,serum total cholesterol(TC),glycosylated hemoglobin(GHb),total triglyceride(TG),malondialdehyde(MDA),superoxide dismutase(SOD)and catalase(CAT)were measured.Results:The content of salidroside in RRE was significantly decreased after irradiation(P<0.05),and the content of loxavir was significantly increased at 5,10 and 20 kGy(P<0.05).Tyrosol was detected at 20 kGy.Compared with the model group,the body weight of mice was given a gavage with irradiation RRE was increased,the food intake,water intake and blood glucose level decreased,and the food utilization rate increased significantly(P<0.05).The levels of TC,GHb,TG and MDA were significantly decreased(P<0.05),while the activities of SOD and CAT were significantly increased(P<0.05).The phenomenon of organ swelling was improved,and the effect was better at 20 kGy,and there was a significant difference with other irradiation doses(P<0.05).Conclusion:The composition and content of RRE compounds changed after irradiation,and RRE can reduce the level of diabetes in type 1 diabetic mice before and after irradiation.The irradiation dose of 20 kGy is effective.
作者
曲孟
常艳秋
齐欣
崔承弼
QU Meng;CHANG Yan-qiu;QI Xin;CUI Cheng-bi(Agricultural College of Yanbian University,Yanji,Jilin 133002,China;School of Pharmacy,Yanbian University,Yanji,Jilin 133002,China)
出处
《食品与机械》
北大核心
2022年第11期160-167,共8页
Food and Machinery
基金
吉林省科技发展技术项目(编号:20220202041NC)。
关键词
1型糖尿病
辐照
红景天
降血糖
type 1 diabetes
irradiation
Rhodiola rosea
hypoglycemic
