摘要
目的研究血必净注射液对体外循环后急性肺损伤大鼠肺泡Ⅱ型上皮细胞凋亡相关微RNA(miRNA)表达的影响。方法采用生物信息学技术筛选与肺泡Ⅱ型上皮细胞凋亡相关的候选miRNA;30只雄性SD大鼠(20~22周龄,体重450~500 g)按随机数字表法将其分为假手术组、体外循环组和血必净组,假手术组不建立体外循环模型,体外循环组和血必净组建立体外循环模型。假手术组于血管穿刺前2 h、体外循环组于体外循环前2 h经腹腔注射4 ml/kg的生理盐水。血必净组于体外循环前2 h经腹腔注射血必净注射液4 ml/kg,体外循环结束后4 h放血处死大鼠,取肺组织标本,采用大鼠肺湿/干重比(W/D)和肺泡损伤数定量评价指标(IQA)评价肺损伤程度;rT-PCR观察血必净对体外循环后急性肺损伤肺泡Ⅱ型上皮细胞中候选miRNA表达的影响。结果通过生物信息学分析筛选得到与大鼠肺泡Ⅱ型上皮细胞凋亡相关的候选miRNAs 5条:rno-miR-17-5p、rno-miR-34a-5p、rno-miR-26a-5p、rno-miR-21a-5p和rno-miR-375-3p。与假手术组比较,体外循环组W/D和IQA升高,差异有统计学意义(P<0.05)。与体外循环组比较,血必净组W/D、IQA下降,差异有统计学意义(P<0.05);与假手术组比较,体外循环组大鼠肺泡Ⅱ型上皮细胞miRNA-17-5p表达下调,而miRNA-34a-5p表达上调,差异有统计学意义(P<0.05);与体外循环组比较,血必净组肺泡Ⅱ型上皮细胞miRNA-17-5p表达上调,差异有统计学意义(P<0.05),而miR-34a-5p表达下调,差异无统计学意义(P>0.05),rno-miR-26a-5p、rno-miR-21a-5p和rno-miR-375-3p在三组中差异无统计学意义(P>0.05)。结论血必净注射液可能通过上调miRNA-17-5p对体外循环后急性肺损伤起到治疗作用,初步揭示血必净注射液治疗体外循环后急性肺损伤的分子机制。
Objective To study the effect of Xuebijing Injection on the expression of apoptosis-related microRNA(miRNA)in alveolar typeⅡepithelial cells of cardiopulmonary bypass-induced acute lung injury rats.Methods The candidate miRNAs related to apoptosis of alveolar typeⅡepithelial cells were screened by bioinformatics technology;30 male SD rats(20 to 22 weeks old,body weight 450 to 500 g)were divided into three groups according to the random number table method.Sham operation group,cardiopulmonary bypass group and Xuebijing group.In the sham operation group,4 ml/kg of normal saline was injected intraperitoneally two hours before the arterial and venous puncture,and in the cardiopulmonary bypass group two hours before the cardiopulmonary bypass.In Xuebijing group,4 ml/kg of Xuebijing Injection was injected intraperitoneally two hours before cardiopulmonary bypass,and the rats were sacrificed by bloodletting four hours after cardiopulmonary bypass,and lung tissue samples were taken.Lung wet/dry weight ratio(W/D)and alveolar injury quantitative assessment index(IQA)were used to evaluate the degree of lung injury.The effect of Xuebijing Injection on the expression of candidate miRNAs in alveolar typeⅡepithelial cells of cardiopulmonary bypass-induced acute lung injury was observed by RT-PCR.Results Five miRNAs related to apoptosis of alveolar typeⅡepithelial cells were screened by bioinformatics analysis rno-miR-17-5p,rno-miR-34a-5p,rno-miR-26a-5p,rno-miR-21a-5p,and rno-miR-375-3p.Compared with Sham operation group,W/D and IQA increased in cardiopulmonary bypass group,and the difference was statistically significant(P<0.05)Compared with the cardiopulmonary bypass group,the W/D and IQA of the Xuebiying group were significantly decreased,and the differences were statistically significant(P<0.05).The expression of miRNA-17-5p in alveolar typeⅡepithelial cells of cardiopulmonary bypass group was lower than that of sham operation group,and the difference was statistically significant(P<0.05),while the expression of miRNA-34a-5p was up-regulated(P<0.05);Compared with the cardiopulmonary bypass group,the expression of miRNA-17-5p in alveolar typeⅡepithelial cells in the XBJ group was up-regulated,and the difference was statistically significant(P>0.05),while the expression of miR-34a-5p was down-regulated,but there was no statistical difference between the two groups(P>0.05).Conclusion Xuebijing Injection may play a therapeutic role in the treatment of cardiopulmonary bypass-induced acute lung injury by up-regulating miRNA-17-5p,and initially reveal the molecular mechanism of Xuebijing injection in the treatment of CPB-Induced acute lung injury.
作者
徐朝军
张胜康
张怡
周代勇
宋岚
XU Zhaojun;ZHANG Shengkang;ZHANG Yi;ZHOU Daiyong;SONG Lan(Department of Cardiothoracic Surgery,the First Affiliated Hospital,Hunan University of Chinese Medicine,Hunan Province,Changsha 410007,China;Hunan University of Chinese Medicine,Hunan Province,Changsha 410208,China)
出处
《中国医药导报》
CAS
2022年第31期22-26,43,共6页
China Medical Herald
基金
湖南省教育厅科学研究重点项目(20A364)
湖南中医药大学中医学国内一流建设学科项目(湘教通2018-469号)。
