摘要
为提高中华鳖背甲中胶原蛋白的提取率,探索鳖甲脱钙工艺条件,以盐酸为脱钙剂,在超声波辅助处理下对鳖甲脱钙条件进行优化。以脱钙率和胶原蛋白迁出率为评价指标,考察盐酸浓度、料液比、脱钙时间、脱钙温度、超声间隙工作时间和超声功率6个因素对鳖甲脱钙效果的影响,确定单因素最优水平。在此基础上,采用响应面Box-Behnken设计对鳖甲脱钙工艺条件进行优化并建立数学模型,并通过紫外光谱扫描、SDS-PAGE图谱、傅立叶红外扫描、氨基酸分析和圆二色光谱结果探讨超声辅助脱钙过程对胶原蛋白结构性质的影响。结果表明,超声波辅助鳖甲脱钙最佳工艺条件为:盐酸浓度0.8 mol/L、温度25℃、料液比1:40(g:mL)、脱钙时间60 min、超声功率145 W、超声间隙30 s/30 s,在此条件下鳖甲脱钙率为64.97%±1.03%,胶原蛋白迁出率为3.22%±0.84%。超声脱钙处理未引起鳖甲胶原蛋白的亚基组成、三螺旋结构和二级结构组成等结构特征的明显变化。因此超声波辅助盐酸对鳖甲脱钙有较好的效果,这将为鳖甲进一步提取胶原蛋白的研究提供基础。
In order to determine the technological conditions for decalcification of the carapace from the Chinese soft-shelled turtle(Pelodiscus sinensis),the decalcification conditions were optimized under ultrasonic assisted treatment with hydrochloric acid as a decalcifying agent.The decalcification rate and collagen migration rate were employed as evaluation indicators to investigate the effects of six factors including hydrochloric acid concentration,material-to-liquid ratio,decalcified time,decalcified temperature,working time of ultrasonic gap and ultrasonic power.In basis of optimized single factors,the Box-Behnken design in the response surface was used to optimize the process conditions of turtle carapace decalcification and establish a mathematical model.The effects of ultrasound-assisted decalcification process on the structural properties of collagen were investigated through biochemical character such as Ultraviolet spectrum scanning,SDS-PAGE spectrum,Fourier infrared scanning,amino acid analysis and Circular dichroism spectrum.The results showed that the best process conditions for ultrasonic-assisted decalcification of the carapace were:0.8 mol/L of hydrochloric acid concentration,1:40(g/mL)of material-to-liquid ratio,60 min of decalcified time,145 W of ultrasound and 30 s/30 s of ultrasonic gap.Under these conditions,the decalcification rate was 64.97%±1.03%,and the collagen migration rate was 3.22%±0.84%.The results showed that the subunit composition,triple helix structure and secondary structure of carapace collagen remained unchanged.Therefore,ultrasound-assisted hydrochloric acid exhibited a good effect on the decalcification of the carapace of the Chinese soft-shelled turtle,which would provide a basis for further study concerning extraction of collagen from the carapace of the Chinese soft-shelled turtle.
作者
赵琼瑜
胡鉴
李彩燕
徐树杰
宋伟
ZHAO Qiongyu;HU Jian;LI Caiyan;XU Shujie;SONG Wei(College of Biological and Environmental Sciences,Zhejiang Wanli University,Ningbo 315000,China)
出处
《食品工业科技》
CAS
北大核心
2022年第22期39-51,共13页
Science and Technology of Food Industry
基金
浙江省基础公益研究计划项目(LGN19C190006)
浙江省农业(水产)新品种选育重大科技专项(2021C02068-8-3)
宁波市科技创新2025重大专项(2021Z009)。
关键词
中华鳖
背甲
脱钙
盐酸
超声波辅助
胶原蛋白
生化特征
Pelodiscus sinensis
carapace
decalcification
hydrochloric acid
ultrasonic-assisted
collagen
biochemical characterization
