摘要
目的研究老鹳草巴布剂治疗类风湿性关节炎的药效作用。方法将30只雌性SD大鼠随机分为对照组、模型组、空白制剂组、伤湿止痛膏(阳性药)组、老鹳草巴布剂(含没食子酸3.40 mg·g^(-1))组,每组6只。除对照组外,其余各组构建佐剂性关节炎大鼠模型:于每只大鼠右后足足趾皮内注射弗氏完全佐剂0.1 mL致炎,对照组大鼠相同部位注射同等体积生理盐水,第7天再次注射加强免疫。对照组及模型组不做干预,阳性药组、老鹳草巴布剂组、空白制剂组分别连续21 d于大鼠右后大腿关节处贴敷伤湿止痛膏、老鹳草巴布剂、空白巴布剂。观察大鼠体质量及足趾肿胀度的变化;采用酶联免疫吸附(ELISA)法测定不同组别大鼠血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、血管内皮生长因子A(VEGF-A)及白细胞介素-17(IL-17)水平;HE染色观察各组大鼠膝关节滑膜病理变化。结果对照组大鼠健康状态良好,毛发顺滑有光泽;模型组大鼠较对照组可见活动、饮食减少,毛发略微发黄,足趾肿胀,关节呈急性炎症表现,表面皮肤发红发亮充血,活动受限,但无死亡;老鹳草巴布剂组、伤湿止痛膏组大鼠状态有所改善。HE染色结果表明,对照组大鼠关节结构正常;模型组、空白制剂组大鼠膝关节可见滑膜细胞增生、炎性细胞浸润并伴有血管翳生成,软骨被侵蚀;与模型组比较,老鹳草巴布剂组与伤湿止痛膏组大鼠踝关节病变情况不同程度得到改善。与模型组比较,老鹳草巴布剂组和伤湿止痛膏组大鼠足肿胀度显著降低(P<0.05),血清IL-6、TNF-α、VEGF-A、IL-17水平明显降低(P<0.05)。结论老鹳草巴布剂抗类风湿性关节炎的作用良好。
Objective To study the effect of Geranium wilfordii cataplasm in treatment of rheumatoid arthritis.Methods Thirty female SD rats were randomly divided into control group,model group,blank preparation group,Shangshi Zhitong ointment(positive drug)group,and Geranium wilfordii cataplasm group(containing gallic acid 3.40 mg·g^(-1))with six rats in each group.Except for the control group,the other groups were used to establish the rat model of adjuvant arthritis:0.1 mL Freund’s complete adjuvant was injected intradermally into the right posterior toe of each rat to cause inflammation,and the control group was injected with the same volume of normal saline in the same part,and then injected again on the 7th day to enhance immunity.The conrol group and the model group were not intervened.The positive drug group,the Geranium wilfordii cataplasm group and the blank formulation group were respectively applied Shangshi Zhitong ointment,Geranium wilfordii cataplasm,blank poultice at the right posterior thigh joint of each rat for 21 days.The changes of body weight and toe swelling degree of rats were observed.Enzymelinked immunosorbent assay was used to determine the serum levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),vascular endothelial growth factor A(VEGF-A)and interleukin-17(IL-17)in different groups of rats.HE staining was used to observe the pathological changes of the knee joint synovium of the rats in each group.Results The rats in the control group were in good health with smooth and glossy hair.Compared with the control group,the rats in the model group showed decreased activity and diet,slightly yellowing hair,swelling of toes,acute inflammation of joints,red,shiny and congested surface skin,limited activity,but no death.The state of rats in Geranium wilfordii cataplasm group and Shangshi Zhitong ointment group was improved.The results of HE staining showed that the joint structure of rats in the control group was normal.Synovial cell proliferation,inflammatory cell infiltration,pannus formation and cartilage erosion were observed in the knee joint of rats in model group and blank preparation group.Compared with the model group,the ankle joint lesions of rats in Geranium wilfordii cataplasm group and Shangshi Zhitong ointment group were improved to different degrees.Compared with model group,the degree of foot swelling of rats in Geranium wilfordii cataplasm group and Shushizhitong ointment group was significantly decreased(P<0.05),and the levels of serum IL-6,TNF-α,VEGF-A and IL-17 were significantly decreased(P<0.05).Conclusion Geranium wilfordii cataplasm has good anti-rheumatoid arthritis effect.
作者
杨小双
宋信莉
甘莹莹
陈欢欢
石佳楠
刘兴德
YANG Xiaoshuang;SONG Xinli;GAN Yingying;CHEN Huanhuan;SHI Jianan;LIU Xingde(School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;National Engineering Research Center of Miao's Medicines,Guiyang 550025,China;Guizhou Chinese Medicine Processing and Preparation Engineering Technology Research Center,Guiyang 550025,China)
出处
《药物评价研究》
CAS
2022年第9期1816-1821,共6页
Drug Evaluation Research
基金
贵州省特色功能食品与中药制剂开发攻关大平台黔教合(KY字[2020]006)
贵州中医药大学药用高分子材料研究中心(贵中医党办发[2019]70号)
贵州中医药大学博士启动基金(贵中医博士启动[2021]14号)
贵州省国内一流学科建设项目(GNYL[2017]008)
贵州省药物新剂型新工艺科技创新人才团队项目(黔科合平台人才[2017]5655)。
