摘要
【目的】探究米槁(Cinnamomum migao)果实不同发育时期根际土壤可培养真菌群落组成的动态变化,为米槁根际土壤有益真菌的利用及有害微生物的防治提供参考。【方法】基于形态学与分子系统发育分析相结合的方法,对米槁根际可培养根际真菌进行分离鉴定。【结果】从米槁(Cinnamomum migao)果实不同发育时期根际土壤共获得529株真菌,初步鉴定为3门35科57属111种。优势菌门为子囊菌门(Ascomycota),优势菌科为肉座菌科(Hypocreaceae)和发菌科(Trichocomaceae);优势菌属为木霉属(Trichoderma)和青霉属(Penicillium)。米槁果实不同生长时期真菌群落组成存在一定差异,幼果期分离鉴定的真菌为31属53种,优势菌属为丛赤壳属(Nectria),占11.90%;膨大期分离鉴定得到的真菌36属64种,优势菌属为木霉属(Trichoderma)和青霉属(Penicillium)分别占16.99%和16.34%;成熟期分离鉴定出的真菌为16属31种,优势菌属为木霉属(Trichoderma),占41.90%。根际可培养真菌群落的多样性指数(Shannon)、优势度指数(Simpson)、丰富度指数(Margalef)和均匀度指数(Pielou)均表现为膨大期﹥幼果期﹥成熟期。【结论】米槁根际土壤可培养真菌多样性丰富,果实成熟期根际可培养真菌多样性明显低于膨大期和幼果期,病原真菌镰孢属(Fusarium)也有相同的变化趋势,推测是病原拮抗菌木霉属(Trichoderma)在果实成熟期大量繁殖,抑制了镰孢属(Fusarium)进一步繁殖导致。
【Objective】The dynamic changes of the fungal community composition in the rhizosphere soil at different development stages of Cinnamomum migao fruit are explored, which provides reference for the utilization of beneficial fungus in the rhizosphere soil of C. migao and the control of harmful microbes.【Method】Based on the method of combining morphology and molecular phylogeny analysis, the cultivable rhizosphere fungus of C. migao were isolated and identified. 【Result】A total of 529 fungi were obtained, which were initially identified as 111 species in 57 genera, 35 families, 3 phyla. At the phylum level, the dominant fungi was Ascomycota;At the family level, the dominant fungi were Hypocreaceae and Phytobacteria;At the genus level, the dominant fungi were Trichoderma and Penicillium. There were some differences in the composition of fungal communities at the three stages. Among them, The fungi isolated and identified at the young fruit stage belonged to 31 genera and 53 species, the dominant genus was Nectria(11.90%);The fungi isolated and identified at the swelling stage were 36 genera and 64 species, the dominant genus were Trichoderma(16.99%) and Penicillium(16.34%);The fungi isolated and identified at maturity stage were 16 genera and 31 species, and the dominant genus was Trichoderma(41.90%). The Shannon diversity index, Simpson index, Margalef index and Pielou index of the rhizosphere cultivable fungal community all showed as the swelling stage﹥young fruit stage﹥maturity stage.【Conclusion】The rhizosphere soil of C. migao had a rich diversity of cultivable fungi, and it at maturity stage was significantly lower than at the swelling stage and the young fruit stage. The pathogenic fungus Fusarium also had the same changing trend. It was speculated that because the pathogenic antagonist Trichoderma proliferates in large numbers during the fruit ripening stage, the further reproduction of Fusarium was inhibited.
作者
田秀
黄路婷
谢元贵
刘济明
吴婷婷
TIAN Xiu;HUANG Lu-ting;XIE Yuan-gui;LIU Ji-ming;WU Ting-ting(Forestry College,Guizhou University,Guiyang 550025,China;Forest Service Center,Honghuagang Forestry Bureau,Zunyi,Guizhou 563000,China;Guizhou Academy of Sciences,Guiyang 550001,China)
出处
《西南农业学报》
CSCD
北大核心
2022年第8期1848-1854,共7页
Southwest China Journal of Agricultural Sciences
基金
贵州省科技计划项目[黔科合支撑(2019)2774号、黔科合成果(2021)一般130、黔科合基础-ZK(2021)一般100]
贵州省土地绿色整治工程研究中心[黔发改投资(2019)886号]
贵州科学院资助项目[黔科院人才(2019)07号、黔科院科专合字(2019)06号]
贵阳国家高新区科技计划项目(GXYF-2017-003,GXYF-2018-003)。
关键词
根际真菌
多样性
米槁
Rhizosphere fungi
Diversity
Cinnamomum migao
