摘要
上海郊区养殖的罗氏沼虾出现了头部“白点病”,造成大量沼虾死亡。患病罗氏沼虾的明显临床症状是位于额角基部的造血组织呈白色不透明状。组织病理学检查表明,病虾造血组织细胞出现异常的嗜碱性包涵体。超微病理研究发现,其造血细胞的细胞质被大量病毒颗粒侵染,该病毒颗粒呈二十面体结构,直径大小为(153±8)nm。聚合酶链式反应(PCR)检测表明,该病毒三磷酸腺苷(ATP)酶基因与十足目虹彩病毒(DIV1)基因序列一致性达100%。根据综合病理和分子检测结果,确认此次上海地区养殖罗氏沼虾“白点病”是由十足目虹彩病毒(DIV1)导致的。文章可为罗氏沼虾“白点病”的诊断和病害防控提供理论依据。
A“white spot disease”on the head of Macrobrachium rosenbergii has been observed in the suburbs of Shanghai which caused a large number of deaths.The obvious clinical sign was the white opaque appearance of the hematopoietic tissue located in the base of the rostrum.Histopathological examination showed that abnormal basophilic inclusion bodies appeared in the hematopoietic cells of the diseased shrimp.Ultrastructural pathology studies revealed that the cytoplasm of the hematopoietic cells was invaded by a large number of viral particles.The virion has an icosahedral structure with a size of(153±8)nm in diameter.PCR results showed the ATPase gene of the virus was 100%identical to the decapod iridescent virus(DIV1)gene sequence.Based on the results of pathology and molecular detection,it was confirmed that the“white spot disease”of M.rosenbergii was caused by DIV1 in the suburban area of Shanghai.
作者
王文雁
王建刚
王元
WANG Wenyan;WANG Jiangang;WANG Yuan(Fisheries Technology Promotion Station of Fengxian District,Shanghai 201499,China;East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China)
出处
《水产科技情报》
2022年第5期290-295,共6页
Fisheries Science & Technology Information
基金
中央级公益性科研院所基本科研业务费专项(2019M03)
中国水产科学研究院基本科研业务费(2020TD41)。
关键词
罗氏沼虾
虹彩病毒
疾病诊断
防控
Macrobrachium rosenbergii
iridescent virus
disease diagnosis
prevention
