摘要
重金属超积累植物是一种高度特化的植物资源,其高效吸收、转运和耐受重金属的机理解析将极大促进植物修复技术发展和农作物的遗传改良。以仅在中国发现的景天科超积累植物新种伴矿景天(Sedum plumbizincicola)为研究对象,我们克隆了一个重金属相关异戊二烯基植物蛋白基因SpHIPP45。表达SpHIPP45的酵母转化子对镉(Cd)的耐受性得到极大增强,但对其他金属离子如锌(Zn)、锰(Mn)、铜(Cu)胁迫的耐受性没有影响。由于SpHIPP45酵母转化子中Cd的含量变化不大,暗示其不是通过减少细胞中Cd含量来介导Cd抗性。进一步研究发现,表达SpHIPP45后,参与内质网应激反应的靶基因在SpHIPP45酵母转化子中转录上调,暗示SpHIPP45可能通过调控内质网应激反应介导Cd耐受性。
Metal hyperaccumulators, series of highly specialized plants, are known for their highly efficient metal uptake, translocation and tolerance. Elucidation of the underlying mechanisms would essentially facilitate phytoremediation and crop improvement. Using a novel cadmium(Cd) hyperaccumulator Sedum plumbizincicola that specifically found in eastern China, we identified a heavy metal-associated isoprenylated plant protein(HIPP) gene Sp HIPP45. Ectopic expression of Sp HIPP45 in yeast enhanced Cd tolerance, but did not enhance the tolerance to other metals’ stress such as zinc, manganese and copper. We did not find much change in the Cd content in yeast addressing SpHIPP45, indicating that it did not mediate Cd tolerance by reducing the content of Cd in cells. However, the yeast transformants showed higher resistance to endoplasmic reticulum stress(ERS) introduced by tunicamycin. The upregulation of mRNA expression levels of target genes involved in the ERS response further verified that SpHIPP45 mediates Cd tolerance by regulating the ERS response.
作者
张培红
张仕泽
张治远
李明悦
刘奕君
张雪洁
白宁宁
马敏
彭佳师
ZHANG Peihong;ZHANG Shize;ZHANG Zhiyuan;LI Mingyue;LIU Yijun;ZHANG Xuejie;BAI Ningning;MA Min;PENG Jiashi(School of Life and Health Sciences,Hunan University of Science and Technology,Xiangtan,Hunan 411201,China;Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization,Hunan University of Science and Technology,Xiangtan,Hunan 411201,China;Key Laboratory of Ecological Remediation and Safe Utlization of Heavy Metal-Polluted Soils,Hunan University of Science and Technology,Xiangtan,Hunan 411201,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2022年第7期1346-1352,共7页
Plant Physiology Journal
基金
湖南省教育厅资助科研项目(20K055)
国家自然科学基金(U20A2024)。
关键词
镉
伴矿景天
内质网应激反应
超积累植物
重金属相关异戊二烯基植物蛋白
cadmium
Sedum plumbizincicola
endoplasmic reticulum stress response
hyperaccumulator
heavy metal-associated isoprenylated plant protein
