摘要
复方蛇床子洗剂是由临床验方制成的常用院内制剂,其临床疗效确切,但其药效物质基础尚未阐明,本文采用超高效液相色谱-高分辨飞行时间质谱联用技术(UPLC-QTOF-MS/MS)对其所含的化学成分进行了较全面的表征。采用电喷雾(ESI)正、负离子电离模式,根据精确分子质量、碎片离子特征、色谱保留时间信息鉴别化合物,共鉴定出118个化合物,包括45种生物碱、21种香豆素及色原酮类、19种黄酮及其苷类、14种三萜皂苷类、10种蒽醌和9种有机酸类化合物。结果表明,UPLC-Q-TOF-MS/MS技术可快速、灵敏地鉴定复方蛇床子洗剂中的化学成分,为探讨该制剂的药效物质基础及进一步的制剂质量控制研究提供了有益参考。
Fufang Shechuangzi Xiji(Lotion) is a common hospital preparation made from clinical prescriptions.Its clinical efficacy is accurate, yet its pharmacodynamic material basis has not been clarified. An ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS/MS)technique was developed to identify the chemical constituents of Fufang Shechuangzi Xiji(Lotion). The chemical constituents of Fufang Shechuangzi Xiji(Lotion) were scanned by electrospray ionization(ESI) source in positive and negative ion modes. As a result, a total of 118 compounds were identified and characterized via reference standards and by comparing mass spectrometry data with literature, including 45 alkaloids, 21 coumarins and chromones, 19 flavonoids, 14 saponins, 10 anthraquinones and 9 organic acids. As a result, UPLC-QTOF-MS/MS technology can quickly and sensitively identify the chemical constituents of Fufang Shechuangzi Xiji(Lotion),which provides a useful reference for exploring the pharmacodynamic material basis and further quality control study of the preparation.
作者
成小红
蒋丽
董兴婷
康秀琳
刘丽芳
CHENG Xiaohong;JIANG Li;DONG Xingting;KANG Xiulin;LIU Lifang(Hospital for Skin Diseases,Chinese Academy of Medical Sciences,Nanjing 210042;Department of Pharmacognosy,School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China)
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2022年第4期452-466,共15页
Journal of China Pharmaceutical University
