摘要
以乙醇为溶剂提取牛蒡根皮中的多酚,用甲醇-乙酸乙酯对牛蒡根皮进行脱脂处理后,以水为溶剂提取牛蒡根皮中的多糖。分别将牛蒡根皮的多酚粗提物、多糖粗提物按照400 mg/kg的添加量添加于海兰褐壳蛋鸡基础日粮中,按照牛蒡根皮多酚粗提物组、牛蒡根皮多糖粗提物组、对照组(饲喂基础日粮)喂养40周龄的海兰褐壳蛋鸡,喂养期为30 d,分析日粮中添加牛蒡根皮多酚粗提物、多糖粗提物对海兰褐壳蛋鸡产蛋性能、蛋品质、血液生化指标的影响。结果表明,日粮中分别添加牛蒡根皮多酚粗提物、多糖粗提物均能提高海兰褐壳蛋鸡的产蛋性能,对总产蛋质量的影响显著(P<0.05);与对照相比,日粮中分别添加牛蒡根皮多酚粗提物、多糖粗提物的软蛋率、破蛋率、畸形蛋率极显著(P<0.01)降低,但蛋壳质量分数未表现出显著差异,多酚粗提物组的哈夫单位显著提高(P<0.05);日粮中添加牛蒡根皮多糖粗提物的海兰褐壳蛋鸡增长的体质量较对照显著(P<0.05)提高6.39%,日粮中添加牛蒡根皮多酚粗提物的海兰褐壳蛋鸡增长的体质量较对照显著(P<0.05)提高11.43%;与对照相比,日粮中添加牛蒡根皮多酚粗提物显著(P<0.05)降低了海兰褐壳蛋鸡血清中的谷丙转氨酶(ALT)活性、总胆固醇(Total cholesterol, TC)浓度、低密度脂蛋白胆固醇(Low density lipoprotein cholesterol, LDL-C)浓度、磷(Phosphorus, Pi)浓度,极显著(P<0.01)提高了血清中的钙(Ca)浓度,日粮中添加牛蒡根皮多糖粗提物对以上指标的影响不显著。
Ethanol was used as solvent to extract polyphenols from burdock root peel. After degreasing with methanol-ethyl acetate, water was used as solvent to extract polysaccharides. Crude polyphenol extracts and crude polysaccharide extracts were added to the basic diet of Hy-line brown laying hens at the dosage of 400 mg/kg. The 40-week-old Hy-line brown laying hens were fed in crude polyphenol extracts group, crude polysaccharide extracts group and control group, and the feeding period was 30 days. Effects of crude polyphenol extracts and crude polysaccharide extracts on egg laying performance, egg quality and blood biochemical index of Hy-line brown laying hens were analyzed. The results showed that the addition of polyphenols and polysaccharide in the diet could improve the egg laying performance of laying hens, and had significant effect on the total egg weight(P<0.05). Compared with the control, the soft egg rate, broken egg rate and deformed egg rate were significantly reduced in polyphenol and polysaccharide groups(P<0.01), but there was no significant difference in eggshell mass fraction, and the Haugh unit in polyphenol group was significantly increased(P<0.05). Compared with the control group, the increased body weight of Hy-line brown laying hens increased by 6.39% in polysaccharide group and 11.43% in polyphenol group. Compared with the control group, the addition of crude polyphenol extracts in the diet significantly decreased the activity of alanine aminotransferase(ALT), total cholesterol(TC) concentration, low-density lipoprotein cholesterol(LDL-C) concentration, phosphorus(Pi) concentration(P<0.05), and extremely significantly increased the calcium(Ca) concentration in serum(P<0.01). The effect of polysaccharides on the above indicators was not significant.
作者
马明磊
朱强
王波
MA Ming-lei;ZHU Qiang;WANG Bo(School of Food Science and Engineering,Jiangsu Ocean University,Lianyungang 222005,China;Xuzhou City Inspection and Testing Center,Xuzhou 221000,China)
出处
《江苏农业学报》
CSCD
北大核心
2022年第4期1013-1020,共8页
Jiangsu Journal of Agricultural Sciences
基金
江苏省研究生科研与实践创新计划项目(SJCX20_1314)。
关键词
牛蒡根皮
多酚
多糖
产蛋品质
血清生化指标
burdock root peel
polyphenol
polysaccharide
egg quality
serum biochemical indicators
