摘要
To understand the molecular characteristics of the miR477 gene family of grape(Vvi-miR477)and to predict its target genes,the Vvi-miR477 genes were identified from previous small RNA sequencing data,then phylogenetic analysis and prediction of target gene were conducted.The Vvi-miR477 family consists of two precursor sequences and three mature sequences.The miR477 family members were mostly 19-22nt in length.The sequence is relatively conservative.Vvi-MIR477a and Vvi-MIR477b are located on chromosomes 1 and 2,respectively.These precursor sequences can form the typical stable stem-loop structure.Their minimum folding free energy is−39.10 kcal/mol and−50.90 kcal/mol,respectively.The MIR477 family can be divided into three groups.The prediction of target genes showed that Vvi-miR477 targets 26S proteasome,DEAD-box,GRAS family protein,Protein Phosphatase 2C,etc.The GO function of target genes was mainly enriched to six categories.The catabolic process,carboxylic ester hydrolase activity is shown to be high.This study provided a theoretical basis for further exploration of the molecular mechanism of miR477 in grape berry ripening.
出处
《BIOCELL》
SCIE
2022年第4期941-949,共9页
生物细胞（英文）
基金
This work was financially supported by National Key Research and Development Program of China(2018YFD1000105)
Natural Science Foundation of China(NSFC:U1904113)
Program for Innovative Research Team(in Science and Technology)in University of Henan Province(21IRTSTHN021),China.
