摘要
为探讨不同光照周期对脊尾白虾(Exopalaemoncarinicauda)生长、性腺发育以及血淋巴生化成分的影响,本研究挑选体质量为(0.71±0.05)g、体长为(3.62±0.28)cm的脊尾白虾,在水温23~25℃、盐度22~24、pH 7.8~8.1、光照强度为1000 lx的养殖条件下,测定其在5种光照周期(全光照L、16L︰8D、12L︰12D、8L︰16D、全黑暗D,L表示光照时长,D表示黑暗时长)下的生长、性腺发育以及血淋巴生化成分等相关指标。结果显示,在存活率方面,全黑暗组最高[(86.67±5.77)%],其次为12L︰12D组[(83.33±8.82)%],再次为8L︰16D组[(80.00±3.33)%],3组之间无显著差异(P>0.05);在特定生长率方面,8L︰16D组的特定生长率为(2.49±0.20)%/d,显著高于全黑暗组(P<0.05);在蜕壳率方面,8L︰16D组蜕壳率最高,为(6.96±0.50)次/(尾·d)。性腺指数随光照时间的缩短逐渐增大,在光照时间为8L时达到最高值[(2.37±0.04)%],显著高于其他组(P<0.05);8L︰16D组卵巢成熟率为(42.93±4.57)%,显著高于全黑暗组(P<0.05);8L︰16D组卵巢相对长度在实验第30天时显著高于其他组(P<0.05)。8L︰16D组血淋巴蛋白质和胆固醇浓度显著高于全光照组、16L︰8D组和全黑暗组(P<0.05),与12L︰12D组无显著差异(P>0.05);8L︰16D组葡萄糖浓度显著高于全光照组和全黑暗组(P<0.05),与16L︰8D组和12L︰12D组差异不显著(P>0.05);8L︰16D组甘油三酯浓度显著高于其他组(P<0.05)。研究表明,不同光照周期对脊尾白虾生长、性腺发育以及血淋巴生化成分均产生不同程度的影响,8L︰16D是脊尾白虾亲虾促熟和工厂化养殖较为适宜的光照周期。
To investigate the effects of different photoperiods on the growth,gonadal development,and hemolymph biochemical composition in Exopalaemon carinicauda,samples with a mass of(0.71±0.05)g and body length of(3.62±0.28)cm were studied under a water temperature of 23~25℃,salinity of 22~24,pH of 7.8~8.1,and illumination intensity of 1000 lx.The growth,gonadal development,and hemolymph biochemical components were measured under five photoperiods(where L,illumination duration;D,dark duration;whole-day illumination L,16L:8D,12L:12D,8L:16D,and whole-day dark D).Results showed that the D group had the highest numerical survival rate(86.67±5.77)%,followed by 12L:12D group,the survival rate was(83.33±8.82)%,the survival rate of group 8L:16D was(80.00±3.33)%,with no significant difference between these three groups(P>0.05).The specific growth rate of group 8L:16D was(2.49±0.20)%/d,significantly higher than that of group D(P<0.05).The molting rate of group 8L:16D was the highest[(6.96±0.50)sub/(tail·d)].The gonadal index increased gradually with a decrease in illumination time,and reached the highest value at 8L:16D[(2.37±0.04)%],significantly higher than that in other groups(P<0.05).The ovarian maturation rate of group 8L:16D was(42.93±4.57)%,significantly higher than that in group D(P<0.05),and the relative length of the ovary in group 8L:16D was significantly higher than that in other groups on the 30th day(P<0.05).The levels of protein and cholesterol in the hemolymph of group 8L:16D were significantly higher than those of group L,group 16L:8D,or group D(P<0.05),but there was no significant difference with group 12L:12D(P<0.05).The glucose concentration of group 8L:16D was significantly higher than that of group L and group D(P<0.05),but there was no significant difference with group 16L:8D and group 12L:12D(P>0.05).The triglyceride concentration in group 8L:16D was significantly higher than that in the other groups(P<0.05).The results showed that the growth,gonadal development,and hemolymph biochemical components of E.carinicauda were affected by the photoperiods.8L:16D was the most suitable photoperiod for E.carinicauda ripening and factory farming.
作者
庞智予
刘锦龙
张瑜
赵真慧
柳森
赖晓芳
于飞
高焕
PANG Zhiyu;LIU Jinlong;ZHANG Yu;ZHAO Zhenhui;LIU Sen;LAI Xiaofang;YU Fei;GAO Huan(College of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang,Jiangsu 222005,China;Jiangsu Ocean University,Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province,Lianyungang,Jiangsu 222005,China;Jiangsu Marine Biological Industry Technology Collaborative Innovation Center,Lianyungang,Jiangsu 222005,China;Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm,Nanjing,Jiangsu 210014,China;Marine and Fishery Development Promotion Center,Lianyungang,Jiangsu 222005,China)
出处
《渔业科学进展》
CSCD
北大核心
2022年第5期189-196,共8页
Progress in Fishery Sciences
基金
国家重点研发计划资助(“蓝色粮仓”)(2018YFD0901302)
江苏省优势学科建设工程资助项目(PAPD)
江苏海洋大学大学生创新创业训练计划项目(SZ201911641105002)
江苏省研究生科研与实践创新计划项目(SJCX20-1273)共同资助。
关键词
脊尾白虾
光照周期
性腺发育
血淋巴
Exopalaemon carinicauda
Photoperiod
Gonadal development
Hemolymph
