摘要
木贼镰刀菌(Fusarium equiseti)是北方地区重要的植物病原菌之一,筛选对其具有拮抗作用的生防菌资源具有重要的意义。本研究以燕麦(Avena sativa)植株为材料,利用选择性培养基从燕麦根际分离、筛选菌株,采用平板对峙法筛选对木贼镰刀菌具有拮抗作用的菌株,并研究抑菌、促生特性。采用16S rRNA分子生物学技术,确定分离的木贼镰刀菌拮抗菌株的分类学地位。结果表明,经筛选后得到了6株具有拮抗作用的菌株,抑菌率41.84%~91.16%,其中有固氮能力的菌株有5株,固氮酶活性51.82~210.81 nmol·(h·mL)^(−1)(C_(2)H_(4))。具有溶无机磷能力的菌株有5株,溶磷量46.14~135.47μg·mL^(−1),其中菌株DRT-3、菌株DRT-11、菌株DRT-17具有解有机磷的能力,解磷量分别为21.64、31.65和28.64μg·mL^(−1),具有分泌吲哚乙酸(IAA)能力的菌株6株,分泌量41.74~157.28μg·mL^(−1)。经16S rRNA基因序列对优良生防菌株初步鉴定,菌株DRT-2、DRT-8为枯草芽孢杆菌(Bacillus subtilis),菌株DRT-3为蕈状芽孢杆菌(Bacillus mycoides),菌株DRT-11为不动杆菌(Acinetobacter sp.),菌株DRT-17和DRT-18为荧光假单胞菌(Pseudomonas fluorescens)。本研究筛选出对燕麦根腐病致病菌有生防作用的细菌,为后续对燕麦根腐病防治研究奠定了基础。
Fusarium equiseti is one of the most harmful plant pathogens in North China.Isolating and screening the biocontrol bacteria resources for the control of Fusarium equiseti is of great significance.In this study,oat plants were used as materials to isolate and screen strains from the rhizosphere of oat using selective media.The antagonistic strains against Fusarium equiseti were screened using the plate confrontation method,and the growth-promoting properties of the strains were further investigated.The taxonomic status of the strains was confirmed using 16S rRNA molecular biology technology.The results showed that six antagonistic strains were obtained after screening,and the inhibition rates ranged from 41.84%to 91.16%.Among them,five strains had nitrogen fixation ability,and five strains had the ability to dissolve inorganic phosphorus with the activity of nitrogenase ranging from 51.82 to 210.81 nmol·(h·mL)^(−1)(C_(2)H_(4)).Among the strains,only strains DRT-3,DRT-11,and DRT-17 had the ability to dissolve organophosphorus,and the dissolved phosphorus levels were 21.64,31.65,and 28.64μg·mL^(−1),respectively.Six strains possessed the ability to secrete indole-3-acetic acid(IAA),with secretion levels between 41.74 and 157.28μg·mL^(−1).Identification based on 16S rRNA sequencing confirmed that DRT-2 and DRT-8 were Bacillus subtilis,DRT-11 was Acinetobacter sp.,DRT-3 was Bacillus mycoides,and DRT-17 and DRT-18 were Pseudomonas fluorescens.In this study,the screening process identified strains that have biocontrol effects on the pathogens of oat root rot,laying the foundation for subsequent research on the prevention and control of oat root rot.
作者
杨晓蕾
李建宏
姚拓
赵桂琴
窦嘉宁
魏恺全
YANG Xiaolei;LI Jianhong;YAO Tuo;ZHAO Guiqin;DOU Jianing;WEI Kaiquan(College of Grassland Science,Gansu Agricultural University/Key Laboratory for Grassland Ecosystem of Education Ministry,Lanzhou 730070,Gansu,China)
出处
《草业科学》
CAS
CSCD
北大核心
2022年第7期1333-1340,共8页
Pratacultural Science
基金
财政部和农业农村部国家现代农业产业技术体系(CARS-07-C-1)。
关键词
木贼镰刀菌
生防菌
拮抗作用
促生特性
Fusarium equiseti
biocontrol strains
antagonistic effect
growth-promoting properties
