摘要
该研究采用UPLC分别建立绿丝郁金(LSYJ)、温郁金(WYJ)、黄丝郁金(HSYJ)、桂郁金(GYJ)特征图谱及11种化学成分的含量测定方法,并结合特征图谱相似度评价、聚类分析(cluster analysis, CA)、主成分分析(principal component analysis, PCA)、正交偏最小二乘法判别分析(orthogonal partial least square discriminate analysis, OPLS-DA)进行4个基原郁金的质量评价研究。从绿丝郁金、温郁金、黄丝郁金、桂郁金特征图谱中各确定了15、17、15、10个特征峰,指认出其中8个化学成分。除GYJ07,各个基原郁金特征图谱相似度均大于0.854,相似度良好。不同基原郁金11种化学成分含量差异较大,绿丝郁金中莪术烯醇、呋喃二烯酮、异莪术烯醇含量相对较高,温郁金中羟基异吉马呋内酯、莪术二酮、呋喃二烯含量相对较高,黄丝郁金中吉马酮、β-榄香烯、双去甲氧基姜黄素、去甲氧基姜黄素、姜黄素含量相对较高,桂郁金中各成分含量都相对较低。化学计量学分析表明,通过CA、PCA、OPLS-DA均可将4个基原郁金准确地划分为4类,且通过OPLS-DA筛选出呋喃二烯酮、莪术烯醇、莪术二酮、羟基异吉马呋内酯、呋喃二烯5个差异质量标志物。该研究建立的特征图谱及多成分含量测定方法简单快速、重复性好、专属性强,为不同基原郁金的质量控制和区分鉴别提供参考。
In this study, UPLC was used to establish the characteristic chromatograms of Curcumae Radix from different origins(LSYJ, WYJ, HSYJ, and GYJ) and the content determination method of 11 chemical components. The evaluation of characteristic chromatogram similarity, cluster analysis(CA), principal component analysis(PCA), and orthogonal partial least square discriminant analysis(OPLS-DA) were combined to evaluate the quality of Curcumae Radix from four origins. LSYJ, WYJ, HSYJ, and GYJ showed 15, 17, 15, and 10 characteristic peaks, respectively, and 8 of the peaks were identified. The characteristic chromatograms of Curcumae Radix samples(except for GYJ07) from the same origin showed the similarity greater than 0.854. The 11 chemical components had different content among the samples from four origins. Curcumenol, furanodienone, and isocurcumenol were rich in LSYJ;hydroxyisogermafurenolide, curdione, and furanodiene had high content in WYJ;gemacrone, β-elemene, bisdemethoxycurcumin, demethoxycurcumin, and curcumin were rich in HSYJ;all the components had low content in GYJ. The chemometric analysis showed that CA, PCA, and OPLS-DA could accurately classify the four origins of Curcumae Radix into four categories, and five different quality markers, namely furanodienone, curcumenol, curdione, hydroxyisogermafurenolide, and furanodiene, were screened out by OPLS-DA. UPLC in combination with multicomponent content determination is simple, rapid, reproducible, and specific, which can provide reference for the quality control and identification of Curcumae Radix from four origins.
作者
王梦
于梦婷
彭梅梅
殷珍珍
毛春芹
苏联麟
季德
陆兔林
WANG Meng;YU Meng-ting;PENG Mei-mei;YIN Zhen-zhen;MAO Chun-qin;SU Lian-lin;JI De;LU Tu-lin(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2022年第11期2964-2974,共11页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2018YFC1707000)
国家自然科学基金项目(81673598)
江苏省研究生科研与实践创新计划项目(KYCX21_1771)
2021年度江苏省高校“青蓝工程”优秀青年骨干教师培养对象项目
江苏政府留学奖学金项目。
关键词
郁金
基原
特征图谱
含量测定
化学计量学
质量评价
Curcumae Radix
origins
characteristic chromatogram
content determination
chemometrics
quality evaluation
