摘要
目的:探讨九制黄精及其配伍对CUMS抑郁模型小鼠物质能量代谢的影响。方法:以80只KM/SPF级小鼠为试验对象,在雌雄各半和采用28 d不可预期温和刺激复制CUMS抑郁模型小鼠的前提下,随机分为模型组、阳性组、黄精组和配伍组,应用酶联免疫法检测小鼠大脑、小脑、肝、肾、脾中的ICD、α-KGDHC、ATPs和Na;-K;ATPase水平。结果:与阳性组相比,各处理组的作用效果显著(P<0.05),九制黄精可极显著升高雌性小鼠大脑、小脑、肝、肾和脾中的α-KGDHC含量水平(P<0.01),九制黄精及配伍对雌雄小鼠不同器官中的ICD、α-KGDHC、ATPs和Na;-K;ATPase含量水平存在复杂性差异。结论:九制黄精及其配伍对雌雄小鼠物质能量代谢酶水平均有不同程度的作用效果,黄精组对雌性和雄性小鼠抑郁症作用效果最佳。
Objective:The Polygonatum cyrtonema was processed to investigated its effects on material and energy metabolism in CUMS depressed model mice.Methods:80 KM/SPF mice were randomly divided into model group,positive group,processed P.cyrtonema group and compatible group,with male and female were equally divided.CUMS depressed mice model were established by unpredictable mild stress for 28 days.The levels of ICD,α-KGDHC,ATPs and Na;-K;ATPase in brain,cerebellum,liver,kidney and spleen were detected by ELISA.Results:Processed P.cyrtonema and its compatibility had different effects on the enzyme levels of material and energy metabolism in both male and female mice.Compared to the positive group,each treatment group was affected significantly(P<0.05).P.cyrtonema could significantly increase theα-KGDHC content in brain,cerebellum,liver,kidney and spleen of female mice(P<0.01),and complex differences in levels of ICDα-KGDHC,ATPs and Na;-K;ATPase in different organs of male and female mice were observed.Conclusion:Processed P.cyrtonema and its compatibility had different effects on the enzyme levels of marerial and energy metabolism in both male and female mice.P cyrtonema group had the strongest effect on depressed mice.
作者
魏婷
何先元
朱潇宏
汪忠强
樊静娴
喻录容
李昕燃
WEI Ting;HE Xian-yuan;ZHU Xiao-hong;WANG Zhong-qiang;FAN Jing-xian;YU Lu-rong;LI Xin-ran(Chongqing Medical University College of Traditional Chinese Medicine,Chongqing 401331,China;Key Laboratory of Metabolism and Metabolism of Traditional Chinese Medicine in Chongqing,Chongqing 400016,China)
出处
《食品与机械》
北大核心
2022年第7期180-185,207,共7页
Food and Machinery
基金
重庆市科委社会民生项目(编号:cstc2018jscx-msybX0288)
重庆市科卫联合中医药技术创新与应用发展重点项目(编号:2021ZY013872)
重庆医科大学大学生科学研究与创新实验项目(编号:SRIEP201980)。
关键词
九制黄精
CUMS抑郁模型
物质代谢
能量代谢
processed Polygonatum cyrtonema
CUMS depressed model
material metabolism
energy metabolism
