摘要
目的 探讨马钱苷对烧伤大鼠肠黏膜屏障的作用以及对内质网应激(endoplasmic reticulum stress,ERS)途径、上皮细胞凋亡的影响。方法 选取健康雄性SD大鼠,8~12周龄,随机分为对照组、烧伤组和干预组。对照组连续7 d灌胃给予生理盐水(50 mg/kg)后假烧伤处理,烧伤组伤前连续7 d给予生理盐水(50 mg/kg)后烧伤处理,干预组伤前连续7 d灌胃给予马钱苷(50 mg/kg)后烧伤处理。烧伤24 h安乐处死大鼠,手术切取肠组织标本,HE染色观察小肠组织病理改变并评分,透射电镜观察小肠上皮细胞超微结构,流式细胞仪检测小肠黏膜细胞增殖与凋亡情况,Western blot法检测小肠组织中ERS相关蛋白以及凋亡相关蛋白表达变化。结果 3组大鼠的病变、炎症、病变范围、隐窝破坏评分差异有统计学意义,其中干预组各评分低于烧伤组,高于对照组(P <0.05)。烧伤组的小肠组织上皮细胞凋亡情况较干预组严重,且细胞凋亡率明显高于干预组(P <0.05)。干预组的半胱氨酸蛋白酶-3(Caspase-3)、Bax、RNA依赖性蛋白样内质网激酶(PERK)、葡萄糖调节蛋白78(GRP78)和C/EBP同源蛋白(CHOP)表达量明显高于对照组,低于烧伤组,而B淋巴细胞瘤-2蛋白(Bcl-2)高于烧伤组,低于对照组(P <0.05)。结论 马钱苷可减轻烧伤大鼠肠上皮组织病理损害,保护肠黏膜结构,其机制可能与抑制肠上皮细胞ERS途径以及减少细胞凋亡有关。
Objective To investigate the effect of loganin on intestinal mucosal barrier,endoplasmic reticulum stress(ERS)pathway and epithelial cell apoptosis in rats with burn injuries. Method Healthy male SD rats aged 8 ~ 12 weeks were selected and randomly divided into control group,burn group and intervention group. The control group was given normal saline(50 mg/kg)by gavage for 7 consecutive days before sham burn treatment,while the burn group was given normal saline(50 mg/kg)for 7 consecutive days before burned. The intervention group was treated with loganin(50 mg/kg)by gastric gavage for 7 consecutive days before injury. Rats were euthanized at 24hours after burn,and intestinal tissue samples were surgically excised. The pathological changes of small intestinal tissue were observed and scored by HE staining,and the ultrastructure of small intestinal epithelial cells was observed by transmission electron microscope. Flow cytometry was used to detect the proliferation and apoptosis of small intestinal mucosal cells,and Western blot was used to detect the expression changes of ERS-related proteins and apoptosis-related proteins in small intestinal tissues. Results There were statistically significant differences in the scores of lesion,inflammation,lesion extent,and crypt destruction among the three groups. The scores in the intervention group were lower than those in the burn group and higher than those in the control group(P < 0.05).The apoptosis of small intestinal epithelial cells in the burn group was more serious than that in the intervention group,and the apoptosis rate was significantly higher than that in the intervention group(P < 0.05). The protein expressions of Caspase-3,Bax,PERK,GRP 78 and CHOP in the intervention group were significantly higher than those in the control group and lower than those in the injury group,while Bcl-2 was higher than that in the injury group and lower than that in the control group(P < 0.05). Conclusion Loganin could reduce the pathological damage of intestinal epithelium in rats with burn injuries and protect the structure of intestinal mucosa,and its mechanism may be related to inhibiting the ERS pathway of intestinal epithelial cells and reducing apoptosis.
作者
温海玲
孟祥熙
杨景哲
肖长栓
WEN Hailing;MENG Xiangxi;YANG Jingzhe;XIAO Changshuan(Department of Burn and Plastic Surgery,South District Hospital,Affiliated Hospital of Chengde Medical University,Chengde 067000,China)
出处
《实用医学杂志》
CAS
北大核心
2022年第11期1353-1358,共6页
The Journal of Practical Medicine
基金
河北省财政厅项目(编号:LS201805)
2020年承德市科学技术研究与发展计划项目(编号:202006A165)。
关键词
马钱苷
内质网应激
上皮细胞
凋亡
肠黏膜
烧伤
loganin
endoplasmic reticulum stress
epithelial cells
apoptosis
intestinal mucosa
burns
