摘要
甘油-3-磷酸作为渗透压调节物红藻糖苷的合成前体物,参与藻类抗逆应答过程。为了解坛紫菜的抗逆机制,通过超高效液相色谱-质谱联用技术(UPLC-MS)建立甘油-3-磷酸的定量分析方法,分析非生物(温度、盐度和干出)胁迫下的甘油-3-磷酸响应规律。结果表明,温度胁迫1 h后,甘油-3-磷酸的含量快速变化,当温度高于23℃时,甘油-3-磷酸含量与胁迫温度成正比,而在12℃低温下,甘油-3-磷酸的含量反而下降;盐度胁迫促使甘油-3-磷酸的含量快速上升,且高盐胁迫下的变化显著高于低盐环境;而短时间1 h干出胁迫后的甘油-3-磷酸含量略有上升(1.19倍,与对照组相比),但随着干出时间继续延长,甘油-3-磷酸含量总体呈现下降趋势。温度、盐度和干出胁迫后的坛紫菜进行恢复培养后,甘油-3-磷酸含量均趋于对照组水平。综上可知,甘油-3-磷酸可快速响应环境胁迫,这为坛紫菜红藻糖苷抗逆机制的多层次研究奠定了基础。
Glycerol-3-phosphate,as a synthetic precursor of osmotic regulator floridoside,participates in the algal stressdefenses.To understand the stress resistance mechanism ofPyropia haitanensis,ultra performance liquid chromatographycoupled with mass spectrometry was used to analyze the fluctuance of glycerol-3-phosphate inP.haitanensisunder abioticstress(temperature,salinity and drought).The results showed that the content of glycerol-3-phosphate changed rapidlyafter 1 h of temperature stress.As the temperature was higher than 23℃,the content of glycerol-3-phosphate wasproportional to the stress temperature,but decreased when the temperature was lower than 12℃.The content of glycerol-3-phosphate increased rapidly under salinity stress,and the change rate under high salinity stress was significantly higherthan that under low salinity stress.The content of glycerol-3-phosphate increased slightly(1.19 times)after 1 h droughtstress and then showed a downward trend as the drought time continued to 12 h.After abiotic stress,the contents ofglycerol-3-phosphate tended to the control level in the recovery cultural condition.Hence,glycerol-3-phosphate couldrespond to environmental stress,which possibly explain the stress resistance mechanism ofP.haitanensis.
作者
唐欣怡
赵佳丽
陈娟娟
骆其君
陈海敏
杨锐
张鹏
TANG Xinyi;ZHAO Jiali;CHEN Juanjuan;LUO Qijun;CHEN Haimin;YANG Rui;ZHANG Peng(State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Ningbo University,Ningbo,Zhejiang 315211;Zhejiang Mariculture Research Institute,Wenzhou,Zhejiang 325005)
出处
《核农学报》
CAS
CSCD
北大核心
2022年第4期788-794,共7页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金资助项目(41706170)
产业技术体系(CARS-50)
宁波市公益类科技项目(2021s063)。
关键词
坛紫菜
甘油-3-磷酸
非生物胁迫
高效液相色谱质谱联用
Pyropia haitanensis
glycerol-3-phosphate
abiotic stress
high performance liquid chromatography coupledwith mass spectrometry
