摘要
为揭示腐食酪螨对阿维菌素的抗性形成机制,通过RNA-seq技术获得亚致死剂量阿维菌素胁迫下腐食酪螨转录组序列,鉴定到138个差异表达的基因被注释到解毒代谢中,包括4个谷胱甘肽S转移酶基因(GST),12个细胞色素P450基因(P450)和3个羧酸酯酶基因(CarE)。利用RNAi技术沉默差异表达的11个解毒代谢基因后,腐食酪螨对阿维菌素的敏感性发生显著性变化,毒力指数在0.65~6.05。结果显示,GST家族中TPGST7、TPGST45和TPGST49以及P450基因TPCYP3A9、TPCYP3A13和TPCYP4C3均参与腐食酪螨对阿维菌素的抗性形成。此外,葡萄糖醛酸转移酶(GLT)家族基因TPGLT28首次被证实参与腐食酪螨对阿维菌素的抗性形成。
To reveal the farmation mechanism of the resistance of Tyrophagus putrescentiae to abamectin,the transcriptome sequence of Tyrophagus putrescentiae under a sublethal dose of abamectin was obtained by RNA-seq technology.A total of 138 differentially expressed genes were identified and annotated to detoxification metabolism,including four glutathione S transferase genes(GST),12 cytochrome P450 genes(P450)and three carboxylesterase genes(CarE).After silencing the 11 differentially expressed genes by using RNAi technology,the susceptibility of the transcriptome sequence of Tyrophagus putrescentiae to abamectin changed significantly,and the virulence index ranged from 0.65 to 6.05.The results showed that TPGST7,TPGST45 and TPGST49 in GST family and P450 genes TPCYP3A9,TPCYP3A13 and TPCYP4C3 were involved in the formation of resistance of T.putrescentiae to abamectin.In addition,the glucuronosyltransferase(GLT)family gene TPGLT28 was first confirmed to be involved in the formation of abamectin resistance.
作者
曲绍轩
骆昕
徐平
侯子强
李辉平
林金盛
侯立娟
蒋宁
马林
QU Shao-xuan;LUO Xin;XU Ping;HOU Zi-qiang;LI Hui-ping;LIN Jin-sheng;HOU Li-juan;JIANG Ning;MA Lin(Institute of Vegetable Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
出处
《江苏农业学报》
CSCD
北大核心
2022年第3期617-624,共8页
Jiangsu Journal of Agricultural Sciences
基金
财政部和农业农村部国家现代农业产业技术体系项目(CARS-20)
国家自然科学青年基金项目(32001911)
江苏省农业科技自主创新基金项目[CX(21)2021]。
关键词
食用菌害螨
抗性相关基因
RNAI
抗药性
mushroom mite pest
resistance-related gene
RNAi
insecticide resistance
