摘要
目的探讨原发性胆汁性肝硬化(PBC)患者血清成纤维细胞生长因子19(FGF19)、人Klotho蛋白(Klotho)及成纤维细胞肾脏因子受体蛋白4(FGFR4)表达的意义。方法选择中国科学院大学宁波华美医院2017年8月至2020年7月收治的PBC患者63例作为PBC组, 另选择该院同期健康体检者51例作为对照组。采用酶联免疫吸附法测定血清FGF19、Klotho蛋白和FGFR4蛋白表达水平。结果 PBC组血清FGF19[(178.86±21.28)ng/L]、FGFR4蛋白[(2.96±0.47)ng/L]均高于对照组的(69.93±12.12)ng/L和(1.21±0.35)ng/L, Klotho蛋白[(3.25±0.89)μg/L]低于对照组的(9.67±1.53)μg/L(t=32.51、27.98、22.08, 均P < 0.05)。PBC组血清丙氨酸氨基转移酶(ALT)[(84.25±13.24)U/L]、天门冬氨酸氨基转移酶(AST)[(71.82±10.35)U/L]、碱性磷酸酶(ALP)[(278.93±32.45)U/L]均高于对照组的(23.76±3.42)U/L、(23.10±4.53)U/L和(76.81±16.36)U/L(t=31.75、31.26、40.48, 均P < 0.05)。经受试者工作特征(ROC)曲线分析显示, FGF19诊断PBC灵敏度为76.67%, 特异度为61.90%;Klotho蛋白诊断PBC灵敏度为58.82%, 特异度为66.67%;FGFR4蛋白诊断PBC灵敏度为54.55%, 特异度为76.67%。经Pearson分析, FGF19与FGFR4蛋白呈线性正相关(r=0.78, P < 0.05), 而Klotho蛋白与FGFR4蛋白呈线性负相关(r=-0.72, P < 0.05)。结论 PBC患者血清FGF19和FGFR4蛋白水平升高, 而Klotho蛋白水平降低, 且FGF19与FGFR4蛋白呈线性正相关, 而Klotho蛋白与FGFR4蛋白呈线性负相关, 该研究具备显著创新性和科学性。
Objective To investigate the significance of serum fibroblast growth factor 19(FGF19),Klotho and fibroblast growth factor receptor 4(FGFR4)protein expression in patients with primary biliary cirrhosis(PBC).Methods Sixty-three patients with PBC who received treatment in Ningbo Huamei Hospital,University of Chinese Academy of Sciences between August 2017 and July 2020 were included in the PBC group.An additional 51 healthy patients who concurrently received physical examination in the same hospital were included in the control group.Serum FGF19,Klotho and FGFR4 protein expression were determined by enzyme linked immunosorbent assay.Results Serum FGF19 and FGFR4 protein in PBC group were(178.86±21.28)ng/L and(2.96±0.47)ng/L,respectively,which were significantly higher than those in the control group[(69.93±12.12)ng/L,(1.21±0.35)ng/L,t=32.51,27.98,both P<0.05].Klotho protein expression in the PBC group was significantly lower than that in the control group[(3.25±0.89)μg/L vs.(9.67±1.53)μg/L,t=22.08,P<0.05].Serum levels of alanine aminotransferase,aspartate aminotransferase,and alkaline phosphatase in the PBC group were(84.25±13.24)U/L,(71.82±10.35)U/L,(278.93±32.45)U/L,respectively,which were significantly higher than those in the control group[(23.76±3.42)U/L,(23.10±4.53)U/L,(76.81±16.36)U/L,t=31.75,31.26,40.48,all P<0.05].The receiver operating characteristic curve analysis showed that the sensitivity and specificity of FGF19 in the diagnosis of PBC were 76.67%and 61.90%,respectively,they were 58.82%and 66.67%for Klotho protein diagnosis,54.55%and 76.67%for FGFR4 protein.Pearson analysis revealed that there was a positive linear relationship between FGF19 and FGFR4 protein(r=0.78,P<0.05),while there was a negative linear relationship between Klotho protein and FGFR4 protein(r=-0.72,P<0.05).Conclusion In patients with PBC,serum FGF19 and FGFR4 protein levels are increased,while Klotho protein level is decreased.There is a positive linear relationship between FGF19 and FGFR4 protein,and there is a negative linear relationship between Klotho protein and FGFR4 protein.This study is highly innovative and scientific.
作者
张振
干敏芝
Zhang Zhen;Gan Minzhi(Department of Rheumatology and Immunology,Ningbo Huamei Hospital,University of Chinese Academy of Sciences,Ningbo 315010,Zhejiang Province,China)
出处
《中国基层医药》
CAS
2022年第5期660-663,共4页
Chinese Journal of Primary Medicine and Pharmacy
基金
浙江省宁波市自然科学基金项目(2017A610278)。
