摘要
利用单个飞秒激光振荡器作为激发源,搭建了光谱聚焦相干反斯托克斯拉曼散射(CARS)显微成像系统,采用SF10材质的玻璃棒对飞秒脉冲引入啁啾,以提高光谱分辨率,实现单一拉曼位移探测。实验以三聚氰胺为样品,开展了光谱聚焦CARS显微成像研究。同时,在光谱聚焦CARS显微成像系统的泵浦和斯托克斯光路中插入半波片,通过旋转半波片角度,进而改变线偏振激发脉冲的偏振方向。并研究了样品特定区域的共振信号/非共振背景的比值随半波片偏转角度的变化趋势,得到了样品特定区域的共振信号/非共振背景的比值最大时半波片的偏转角度。
The excitation source for a spectrally focused coherent anti-Stokes Raman scattering(CARS) microscopic imaging system is a single femtosecond laser oscillator. The system uses a glass rod composed of SF10 to introduce a chirp into the femtosecond pulse for an enhanced spectral resolution. For single Raman displacement detection, a melamine sample is used to perform experimental spectrally focused CARS microscopic imaging research.Moreover, a half-wave plate is inserted in the pump and Stokes optical path of the spectral focus CARS microscopic imaging system. By changing the half wave, the polarization direction of the linearly polarized excitation pulse is changed, and the variation trend of the ratio of the resonant signal/nonresonant background with respect to the deflection angle of the half-wave plate is studied. Furthermore, the deflection angle of the half-wave plate is determined using the maximum ratio of the resonant signal/nonresonant background in a specific region of the sample.
作者
邸亚盟
刘硕
白振旭
邓岩岩
吕志伟
夏元钦
Di Yameng;Liu Shuo;Bai Zhenxu;Deng Yanyan;LüZhiwei;Xia Yuanqin(Center for Advanced Laser Technology,Hebei University of Technology,Tianjin 300401,China;Hebei Key Laboratory of Advanced Laser Technology and Equipment,Tianjin 300401,China)
出处
《激光与光电子学进展》
CSCD
北大核心
2022年第10期374-381,共8页
Laser & Optoelectronics Progress
基金
国家自然科学基金(61675057,61975050)。
关键词
显微
相干反斯托克斯拉曼散射
显微成像
光谱聚焦
三聚氰胺
非共振背景
microscopy
coherent anti-Stokes Raman scattering
microscopy imaging
spectral focusing
melamine
non-resonant background
