摘要
为探究马缨杜鹃叶片基因表达水平对水淹胁迫的响应。本研究分别以水淹胁迫下不同处理时间的马缨杜鹃幼苗叶片和正常叶片(CK)为试验材料,通过lllumina Hisq高通量测序平台进行转录组测序,分析水淹胁迫下马缨杜鹃幼苗叶片差异表达基因。结果显示,与对照组相比,水淹胁迫10 d后,2006个基因呈现差异表达(其中1067个基因上调,939个基因下调);水淹胁迫20 d后,3564个基因呈现差异表达(其中1698个基因上调,1866个基因下调)。对差异表达基因进行GO富集分析发现,差异表达基因主要富集在代谢过程、细胞过程、单一生物过程、细胞、细胞区域、细胞膜、细胞膜部分、催化活性和结合蛋白。对差异表达基因进行KEGG富集分析发现,差异基因主要富集在植物激素信号转导、淀粉和糖代谢、氨基酸的生物合成及苯丙素的生物合成途径。结果表明水淹胁迫诱导马缨杜鹃叶片相关基因表达,从而响应水淹胁迫,本研究结果为进一步探究马缨杜鹃对水淹胁迫响应分子机制提供初步理论依据。
To explore the response of gene expression level of Rhododendron delavayi Franch to waterlogging stress,the leaves of R.delavayi seedlings under waterlogging stress for different treatment time and normal growth were used as experimental materials in this study.The differentially expressed genes(DEGs)of R.delavayi seedlings leaves were analyzed using lllumina Hisq high-throughput sequencing platform.The results showed that a total of2006 DEGs were obtained from stressed leaves for 10 d compared with CK(1067 up-regulated and 939 down-regulated genes).Moreover,a total of 3564 DEGs were found in stressed leaves for 20 d compared with CK(1698 up-regulated and 1866 down-regulated genes).GO enrichment analysis indicated that DEGs were significantly enriched in metabolic process,cellular process,single-organism process,cell,cell part,membrane,membrane part,catalytic activity and binding.KEGG enrichment analysis indicated that DEGs were mainly enriched in the pathways of plant hormone signal transduction,starch and sugar metabolism,amino acid biosynthesis and phenylpropanoid biosynthesis.These results showed that the stress-related genes were induced by waterlogging stress in R.delavayi leaves.This provides a theoretical basis for revealing the molecular mechanism of R.delavayi responding to waterlogging stress.
作者
段盛光
唐明
唐婧
张习敏
Duan Shengguang;Tang Ming;Tang Jing;Zhang Ximin(Key Laboratory of Plant Physiology and Development Regulation,Guizhou Normal University,Guiyang,550001;Key Laboratory of State Forestry Administration on Biodiversity Conservation in Karst Area of Southwest,Guizhou Normal Lhiiversity,Guiyang,550001;School of Life Science,Guizhou Normal University,Guiyang,550001)
出处
《分子植物育种》
CAS
北大核心
2022年第9期2854-2863,共10页
Molecular Plant Breeding
基金
国家自然科学基金委员会-贵州省人民政府喀斯特科学研究中心项目(U1812401)
中央支持地方科技发展基金([2017]4006)
贵州省林业局科学研究基金([2019]10)
贵州师范大学资助博士科研项目(GZNUD[2019]18)
贵州省科技厅和贵州师范大学联合基金([2017]7353)共同资助。
关键词
马缨杜鹃
转录组测序
水淹胁迫
差异表达基因
Rhododendron delavayi Franch
Transcriptome sequencing
Waterlogging stress
Differentially expressed genes
