摘要
【目的】监测2021年广东地区猪繁殖与呼吸综合征病毒(Porcine Reproductive and Respiratory Syndrome Virus,PRRSV)的分子流行动态,为PRRSV综合防控措施的制定提供参考。【方法】在广东省内不同地区PRRSV检测为阳性的37个规模猪场采集521份患病猪组织样本进行PRRSV检测。对样本进行ORF5基因测序,采用DNAStar分析ORF5核苷酸的同源性及其推导氨基酸的同源性,并用Mega7.0构建基于ORF5基因的系统遗传进化树。【结果】从37个猪场采集的样品PRRSV阳性率为24.4%,猪场阳性率为45.9%。通过测序分析获得17株来自不同猪场的PRRSV ORF5基因序列,遗传进化分析表明所有毒株均属于北美毒株(PRRSV 2),其中有5株属于Lineage 8(JXA1-like和CH-1a-like)谱系,9株属于Lineage 1(NADC30-like和NADC34-like)谱系,3株属于Lineage 3(QYYZ-like)谱系,没有监测到Lineage 5(VR2332-like)谱系的毒株。17株PRRSV毒株的ORF5基因之间核苷酸序列同源性为81.3%~99.5%,推导的氨基酸序列同源性为80.0%~98.3%。【结论】2021年广东省PRRSV流行株以Lineage 1(NADC30-like和NADC34-like)和Lineage 8(JXA1-like和CH-1a-like)谱系为主,占比分别为52.94%和29.41%,Lineage 3(QYYZ-like)谱系降为次要流行株,占比为17.65%。
【Objective】The molecular epidemiological dynamics of porcine reproductive and respiratory syndrome virus(PRRSV)in Guangdong Province in 2021 were monitored in order to provide references for the formulation of comprehensive prevention and control measures for PRRSV.【Method】521 tissue samples of diseased pigs were selected from 37 large-scale farms that were tested positive as PRRSV in different regions of Guangdong Province for ORF5 gene sequencing,then DNAStar was used to analyze the nucleotide homology and deduced amino acid homology of the ORF5 gene,and Mega 7.0 was used to construct ORF5-based phylogenetic tree.【Result】Positive rate of PRRSV was 24.4%in the collected samples,and the positive rate of pig farms was 45.9%.The ORF5 gene sequences of 17 strains of PRRSV from different pig farms were obtained by sequencing.Phylogenetic analysis showed that all the isolates belonged to the North America genotype.Among them,5 strains belonged to the Lineage 8(JXA1-like and CH-1a-like)subgroup,9 strains belonged to the Lineage 1(NADC30-like and NADC34-like)subgroup,3 strains belonged to the Lineage 3(QYYZ-like)subgroup,and no strains of the Lineage 5(VR2332-like)were detected.The nucleotide sequence homology of ORF5 gene of 17 strains was 81.3%-99.3%,and the deduced amino acid sequence homology was 80.0%-98.3%.【Conclusion】The predominate strains of PRRS in Guangdong Province in 2021 belonged to Lineage 1(NADC30-like and NADC34-like)and Lineage 8(JXA1-like and CH-1a-like),accounting for 52.94%and 29.41%,respectively.Lineage 3(QYYZ-like)was reduced to a secondary epidemic strain,accounting for 17.65%.
作者
蒋智勇
楚品品
陈天宝
李春玲
蔡汝健
JIANG Zhiyong;CHU Pinpin;CHEN Tianbao;LI Chunling;CAI Rujian(Institute of Animal Health,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Livestock Disease Prevention/Guangdong Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques,Ministry of Agriculture Rural Affairs,Guangzhou 510640,China)
出处
《广东农业科学》
CAS
2022年第4期97-105,共9页
Guangdong Agricultural Sciences
基金
广东省动物疫病野外科学观测研究站项目(2021B1212050021)
广东省重点领域研发计划项目(2019B020217002)
广东省农业科学院院长基金(202044)。
