摘要
目的:研究小海马Hippocampus japonicas Kaup内生菌Fusarium solani的化学成分。方法:将该菌株进行固体发酵培养,发酵物甲醇提取后的乙酸乙酯萃取部位利用硅胶柱、C18反向硅胶柱、Sephdex-LH.20凝胶柱、MCI柱、制备型液相色谱对其化学成分进行分离纯化,根据物理性质及波谱技术鉴定化合物的结构。结果:从中分离得到15个已知化合物,鉴定为环-(D-脯氨酸-D-苯丙氨酸)(1);环-(L)-脯氨酸-(L)-苯丙氨酸(2);邻羟基苯甲醛(3);4-羟基苯甲酸甲酯(4);乙酰胆碱(5);环-4-羟基-(D)-脯氨酸-(L)-苯丙氨酸(6);环-4-羟基-(L)-脯氨酸-(L)-苯丙氨酸(7);环-(L)-脯氨酸-(L)-亮氨酸(8);2,5-morpholinedione,4-methyl-6-(1-methylethyl)-3-(1-methylpropyl)-(9);(E)-二甲基咖啡酸(10);4-甲氧基-5,6-二甲基-2H-吡喃[2,3-b]吡啶-2-酮(11);3,4-二甲氧基苯乙酮(12);白僵菌素(13);亚油酸(14);9,12十八烷二烯酸甲酯(15)。结论:化合物1-3,6-9,11-12均为首次从该真菌中分离。
Objective:To study the chemical constituents of Fusarium solani,an endophytic fungus from Hippocampus japonicas Kaup.Method:F.solani was firstly cultured by solid fermentation,then the products fermented were extracted by methanol.Finally,the chemical compounds were isolated and purified from the ethyl acetate part of the methanol extract by silica gel column,C18 reversedphase silica gel column chromatography,Sephdex-LH.20,MCI column,and preparative HPLC.The structures of obtained compounds were identified by physical properties and spectroscopic analyses.Result:15 compounds were elucidated as:cyclo-(D-Pro-D-Phe)(1),cyclo-(L-Pro-L-Phe)(2),2-hydroxy-benzaldehyde(3),Methyl 4-Hydroxybenzoate(4),Acetylcholine(5),cyclo-(4-hydroxy-D-prolylL-phenylalanine)(6),cyclo-(4-hydroxy-L-prolyl-L-phenylalanine)(7),cyclo-(L-Pro-L-Leu)(8),2,5-morpholinedione,4-methyl-6-(1-methylethyl)-3-(1-methylpropyl)(9),(E)-3,4-Dimethoxycinnamic acid(10),methoxy-5,6-Dimethyl-2H-pyran[2,3-b]pyridin-2-one(11),ethanone,1-(3,4-dimethoxyphenyl)-(12),beauvericin(13),linoleic acid(14),9,12-octadecadienoic acid(Z,Z)-,methyl ester(15).Conclusion:Compounds 1-3,6-9,and 11-12 were isolated from F.solani for the first time.
作者
李新爱
曹钰镁
巨凤
郭文秀
刘然
郭大乐
张剑光
邓赟
LI Xin-ai;CAO Yu-Mei;JU Feng;GUO Wen-xiu;LIU Ran;GUO Da-lei;ZHANG Jian-guang;DENG Yun(State Key Laboratory of Southwestern Chinese Medicine Resources,Key Laboratory of Standardization for Chinese Herbal Medicine,Ministry of Education,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,Sichuan;Qinzhou Provincial Health School,Qinzhou 535000,Guangxi)
出处
《中药与临床》
2022年第1期1-5,31,共6页
Pharmacy and Clinics of Chinese Materia Medica
基金
国家自然科学基金面上项目(81973460)
四川省重点研发项目(2021YFN0134)
成都中医药大学杏林学者学科人才科研提升计划(BSH2018008)
钦州市科学技术局基础研究项目(20198509)资助。
