摘要
目的:建立一种离子色谱方法同时测定复方电解质注射液中果糖、乳酸钠与抗氧剂亚硫酸氢钠的含量。方法:采用磺酸型聚苯乙烯苯乙烯二乙烯基苯共聚体阳离子交换树脂H型(Aminex HPX-87H)为色谱柱,流动相为0.005 mol·L^(-1)硫酸-乙腈(85∶15),柱温为40℃,检测波长200 nm,流速为0.6 mL·min^(-1),进样量为20μL。结果:果糖和乳酸钠浓度分别在1.60~2.39 mg·mL^(-1)(r=0.9995)、0.24~0.36 mg·mL^(-1)(r=0.9996)呈良好的线性关系,平均回收率分别为99.28%(n=9)、99.75%(n=9),RSD分别为0.66%和0.69%;亚硫酸氢钠浓度在0.00025~0.075 mg·mL^(-1)范围内,其浓度与峰面积呈良好线性关系(r=0.9998);定量限和检测限分别为0.25μg·mL^(-1)和0.10μg·mL^(-1);平均回收率为99.63%,RSD为1.20%(n=12)。结论:本方法准确可靠,重现性好,可快速、高效检测复方电解质注射液中果糖、乳酸钠与亚硫酸氢钠的含量。
Objective:To establish an ion chromatography(IC)method to simultaneously determine contents of fructose,sodium lactate and sodium bisulfite in compound electrolyte injections.Methods:The chromatographic column was Aminex HPX-87H.The mobile phase was 0.005 mol·L^(-1)sulfuric acid-acetonitrile(85∶15)at flow rate of 0.6 mL·min^(-1).The column temperature was 40℃,the injection volume was 20μL and the detection wavelength was 200 nm.Results:The concentrations of fructose and sodium lactate were linear in the ranges of 1.60-2.39 mg·mL^(-1)(r=0.9995)and 0.24-0.36 mg·mL^(-1)(r=0.9996),respectively.The average recoveries were 99.28%(n=9)and 99.75%(n=9),respectively.The concentration of sodium bisulfite was linear in the range of 0.00025-0.075 mg·mL^(-1)(r=0.9998).The limit of detection(LOD)was 0.10μg·mL^(-1)and the limit of quantification(LOQ)was 0.25μg·mL^(-1).The average recovery was 99.63%with RSD at 1.20%(n=12).Conclusion:The method is accurate,reliable and reproducible,and can be used for the rapid and efficient determination of fructose,sodium lactate and sodium bisulfite in compound electrolyte injections.
作者
石洁
朱飞
周静
霍立茹
SHI Jie;ZHU Fei;ZHOU Jing;HUO Liru(Xuzhou Medical University,Xuzhou,Jiangsu 221000,China;Nanjing Critpharma Co.,Ltd.,Nanjing 211112,China)
出处
《药学与临床研究》
2022年第2期123-126,共4页
Pharmaceutical and Clinical Research
