摘要
为探究盐胁迫对不同基因型辣椒的影响,本研究以两个不同耐盐性辣椒品系PE-5、PE-18为试材,采用基质栽培的方式,用150 mmol/L的NaCl胁迫处理,研究盐胁迫对辣椒幼苗渗透调节物质及抗氧化酶活性的影响。结果表明,在盐胁迫条件下,辣椒叶片中的三种渗透调节物质随着盐胁迫时间的延长均表现为先增后减的趋势,且耐盐品种的升高幅度大于不耐盐品种;耐盐品种PE-5游离脯氨酸的含量在处理10 d、15 d时显著高于不耐盐品种PE-18,耐盐品种PE-5可溶性蛋白与可溶性糖的含量在处理5、10、15 d时显著高于不耐盐品种PE-18。在盐胁迫条件下,三种防御酶(超氧化物歧化酶,过氧化物酶,过氧化氢酶)活性随着胁迫时间的延长均表现为逐渐升高的总趋势,且耐盐品种的升高幅度大于不耐盐品种;耐盐品种PE-5三种防御酶的活性在处理5、10、15 d时均显著高于不耐盐品种PE-18。综上,盐胁迫对不同耐盐辣椒品种渗透调节物质的含量及抗氧化酶活性产生了显著影响,耐盐品种PE-5渗透调节物质的含量及防御酶活性均显著高于不耐盐品种PE-18。本研究结果为辣椒的耐盐机制及耐盐育种提供了理论参考。
In order to study the effects of salt stress on different pepper genotypes,this study used two different salt-resistant pepper strains,PE-5 and PE-18,as substrate cultivation and treated with 150 mmol/L NaCl stress to study the effect of salt stress on the osmoregulator and antioxidase activity of pepper seedlings.The results showed that three osmotic adjustment substances in pepper leaves increased first and then decreased with the time of salt stress under salt stress,and the increase of salt-tolerant varieties was larger than salt-intolerant varieties,the content of free proline in salt-tolerant variety PE-5 was significantly higher than that in salt-intolerant variety PE-18 at 10 and 15 days,the content of soluble protein and soluble sugar in salt-tolerant PE-5 was significantly higher than that in salt-intolerant PE-18 at 5,10 and 15 days.Under salt stress,the activities of three defense enzymes all showed a general trend of in creasing gradually with the extension of stress time,and the increasing range of salt-tolerant varietieswas larger than that of non-salt-tolerant varieties,the activities of SOD,POD and CAT in salt-tolerant cultivar PE-5 were significantly higher than those in salt-intolerant cultivar PE-18 at 5,10 and 15 days.In conclusion,salt stress had significant effects on the contents of osmotic adjustment substances and defense enzyme activities in different salt-tolerant pepper varieties.The contents of osmotic adjustment substances and defense enzyme activities in salt-tolerant PE-5 were significantly higher than those in salt-intolerant PE-18.The results of this study provide a theoretical reference for salt tolerance mechanism and salt tolerance breeding of capsicum.
作者
李正丽
张丽
文林宏
张兆辉
Li Zhengli;Zhang Li;Wen Linhong;Zhang Zhaohui(Institute of Horticulture,Guizhou Academy of Agricultural Sciences,Guiyang,550006;Guizhou Academy of Agricultural Sciences Shanmao Horticulture Engineering Technology Co.,Ltd.,Guiyang,550006;Zhuanghang Integrated Experimental Station,Shanghai Academy of Agricultural Sciences,Shanghai,201415)
出处
《分子植物育种》
CAS
北大核心
2022年第5期1658-1663,共6页
Molecular Plant Breeding
基金
贵州省蔬菜现代农业产业技术体系项目(GZCYTX2021-01,筑科合同〔2021〕3-2号,筑科合同〔2021〕5-1号)
茄果类蔬菜优质高效生产模式建立与示范(202003A04)
黔农科院种质资源项目(〔2021〕03号)
黔科合平台人才项目(〔2016〕5671号)共同资助。
关键词
辣椒
盐胁迫
生理生化
Pepper
Salt stress
Physiological and biochemical
