摘要
目的探讨预电针心俞穴对急性心肌缺血大鼠心肌保护作用的可能机制。方法选用雄性清洁级SD大鼠18只,随机分成生理盐水对照组、急性心肌缺血组、预电针心俞穴组。急性心肌缺血组、预电针心俞穴组用背部皮下多点位注射盐酸异丙肾上腺素(ISO)制备急性心肌缺血大鼠模型,预电针心俞穴组在造模前电针双侧心俞穴3 d。采用苏木精-伊红染色法(HE染色法)观察大鼠心肌组织病理学变化;q PCR检测心肌组织SP mRNA和CGRP mRNA表达。结果与急性心肌缺血组相比,预电针心俞穴组可改善大鼠心肌组织病理学变化,抑制心肌组织SP mRNA和CGRP mRNA的表达,心肌缺血损伤程度减轻。结论预电针心俞穴对大鼠急性心肌缺血损伤具有一定的保护作用,可能通过调节SP及CGRP表达对心肌缺血损伤起到保护作用。
Objective:To explore the possible mechanism of pre-electroacupuncture at Xinshu points on myocardial protection in rats with acute myocardial ischemia.Methods:18 male clean SD rats were randomly divided into normal saline control group,acute myocardial ischemia group,and pre-electroacupuncture at Xinshu acupoint group.The acute myocardial ischemia group and the pre-electroacupuncture at Xinshu acupoint group was prepared by subcutaneous injection of isoproterenol hydrochloride(ISO)at multiple points in the back to make rat models of acute myocardial ischemia.The Xinshu acupoint group was given electroacupuncture at Xinshu points on both sides for 3 days before modeling.Hematoxylin-eosin staining method(HE staining method)was used to observe the pathological changes of rat myocardial tissue and q PCR was used to detect the expression of SP mRNA and CGRP mRNA in myocardial tissue.Results:Compared with the acute myocardial ischemia group,the pre-electroacupuncture at Xinshu acupoint group can improve the pathological changes of rat myocardial tissue,inhibit the expression of SP mRNA and CGRP mRNA in myocardial tissue,and reduce the degree of myocardial ischemia injury.Conclusion:The pre-electro-acupuncture at Xinshu points has a certain protective effect on acute myocardial ischemia injury in rats,and may play a protective effect on myocardial ischemia injury by regulating the expression of SP and CGRP.
作者
董亚琴
万隆
黄倩茹
萨喆燕
许金森
DONG Ya-qin;WAN Long;HUANG Qian-ru;SA Zhe-yan;XU Jin-sen(Fujian Academy of Chinese Medical Science,Fuzhou 350003,China)
出处
《云南中医中药杂志》
2022年第1期72-75,共4页
Yunnan Journal of Traditional Chinese Medicine and Materia Medica
基金
国家自然科学青年基金项目(81403490)
福建省卫生计生中青年骨干人才培养项目(2017-ZQN-70)。
关键词
预电针
心俞穴
急性心肌缺血
SP
CGRP
Pre-Electroacupuncture
Xinshu Acupoint
Acute Myocardial Ischemia
SP
CGRP
