摘要
目的探索白头翁皂苷B4对卵巢癌SKOV3细胞株细胞增殖、凋亡及迁移的影响及其机制。方法体外培养卵巢癌SKOV3细胞,利用CCK-8法检测不同浓度白头翁皂苷B4处理SKOV3细胞后其增殖情况,并计算IC_(50)值;流式细胞术检测IC_(50)值浓度下不同时间白头翁皂苷B4处理细胞后的凋亡情况;Transwell法检测IC_(50)值浓度白头翁皂苷B4处理细胞不同时间后细胞迁移和侵袭情况;Western blot检测细胞内相关蛋白表达量变化。结果白头翁皂苷B4能有效抑制SKOV3细胞的增殖,呈现出浓度依赖性,IC_(50)值为(6.08±0.56)μM,且抑制效果强于阳性对照药物顺铂,差异具有统计学意义(P<0.05);流式细胞术显示白头翁皂苷B4处理24 h后SKOV3细胞的凋亡比例为52.3%,且降低了Bcl-xl表达,上调了Bax,cleaved-caspase-3和PARP表达,且与0 h组相比差异具有统计学意义(P<0.05);白头翁皂苷B4能下调N-cadherin的表达,上调E-cadherin表达,进而抑制SKOV3细胞的迁移;白头翁皂苷B4能抑制JAK/STAT3信号通路蛋白的表达。结论白头翁皂苷B4能有效抑制卵巢癌细胞的增殖,并通过调控JAK/STAT3信号通路影响SKOV3细胞凋亡并抑制其发生迁移,白头翁皂苷B4在卵巢癌的治疗及新药开发中具有研究潜力。
Objective To investigate the effects of anemoside B4 on proliferation,apoptosis and migration of ovarian cancer SKOV3 cells through a variety of biological methods,and further to explore its mechanism.Methods Ovarian cancer SKOV3 cells were cultured in vitro.CCK-8 method was used to detect the proliferation of SKOV3 cells treated with different concentrations of anemoside B4,and IC_(50) value was calculated.Flow cytometry was employed to detect the apoptosis of cells treated with IC_(50) concentration of anemoside B4 for different time length;Transwell method was used to detect the migration and invasion of cells treated with IC_(50) concentration of anemoside B4 for different time length.Western blot was used to detect changes in the expression of related proteins.Results Anemoside B4 can effectively inhibit the proliferation of SKOV3 cells,showing a concentration-dependent IC_(50) value of 6.08±0.56μM,and the inhibitory effect is stronger than the positive control drug cisplatin,with statistically significant difference(P<0.05).Flow cytometry showed that anemoside B4 could induce SKOV3 cells apoptosis,reduce Bcl-xl expression,and up-regulate the expression of Bax,cleaved-caspase-3 and PARP.Compared with the group of 0 h,the difference was statistically significant(P<0.05).Crocetin could down-regulate the expression of N-cadherin and up-regulate the expression of E-cadherin,thereby inhibiting the migration of SKOV3 cells.Anemoside B4 could inhibit the expression of JAK/STAT3 signaling pathway proteins.Conclusion Anemoside B4 can effectively inhibit the proliferation of cervical cancer cells,and induce SKOV3 cell apoptosis by regulating the JAK/STAT3 signaling pathway to inhibit their migration.Crocetin has great potential in the research and development of ovarian cancer therapy.
作者
任晶晶
姬艳飞
王静芳
Ren Jingjing;Ji Yanfei;Wang Jingfang(Department of Obstetrics and Gynecology,the Second Hospital of Shanxi Medical University,Taiyuan 030001,China)
出处
《中华内分泌外科杂志》
CAS
2022年第1期89-92,共4页
Chinese Journal of Endocrine Surgery
基金
山西省应用基础研究计划 (201901D111364)。
