摘要
建立用于血浆中补肺活血胶囊活性成分含量测定的超高效液相色谱-串联质谱(UHPLC-MS/MS)方法,并应用于药代动力学研究。SD大鼠灌胃补肺活血胶囊混悬液后,于不同时间点经眼眶静脉丛取血,血浆样品经蛋白沉淀,采用ACQUITY UPLC BEH C_(18)色谱柱(2.1 mm×100 mm,1.7μm)分离,流动相为乙腈(A)-0.1%甲酸水(B),梯度洗脱;在多反应监测模式(MRM)下正、负离子同时测定;采用DAS 3.2.8软件拟合计算药动学参数。结果显示,大鼠灌胃补肺活血胶囊后,血浆中可测得补骨脂苷、异补骨脂苷、毛蕊异黄酮苷、芒柄花苷、补骨脂素、异补骨脂素、3-羟基-9,10-二甲氧基紫檀烷、新补骨脂异黄酮,其药动学参数药时曲线下面积(AUC_(0-t))分别为(3357±1348)、(3555±1696)、(3.03±0.88)、(2.21±0.33)、(1787±522)、(2295±539)、(5.69±1.41)、(3.40±0.75)μg·L^(-1)·h;达峰时间(T_(max))分别为(1.56±0.62)、(1.40±0.70)、(0.21±0.05)、(0.25±0.12)、(0.26±0.11)、(0.34±0.29)、(0.74±0.59)、0.25 h。该检测方法专属性强、重复性好,适用于补肺活血胶囊中补骨脂苷、异补骨脂苷、毛蕊异黄酮苷、芒柄花苷、补骨脂素、异补骨脂素、3-羟基-9,10-二甲氧基紫檀烷、新补骨脂异黄酮在大鼠体内的药动学研究。
An ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)method was established to investigate the pharmacokinetic behaviors of psoralenoside,isopsoralenoside,calycosin-7-glucoside,ononin,psoralen,isopsoralen,methylnissolin,and neobavaisoflavone in rat plasma after oral administration of Bufei Huoxue Capsules.After SD rats were administered with Bufei Huoxue Capsules suspension by gavage,blood samples were collected from the inner canthus at different time points.After protein precipitation,plasma samples were separated on ACQUITY UPLC BEH C_(18)column(2.1 mm×100 mm,1.7μm).The mobile phase consisted of acetonitrile(A)and water(B)containing 0.1%formic acid in gradient elution.The positive and negative ions were measured simultaneously in the multi-reaction monitoring(MRM)mode.The pharmacokinetic parameters were calculated and fitted by DAS 3.2.8.Psoralenoside,isopsoralenoside,calycosin-7-glucoside,ononin,psoralen,isopsoralen,methylnissolin,and neobavaisoflavone were detected in the rat plasma after drug administration,with AUC_(0-t)of(3357±1348),(3555±1696),(3.03±0.88),(2.21±0.33),(1787±522),(2295±539),(5.69±1.41)and(3.40±0.75)μg·L^(-1)·h,and T_(max)of(1.56±0.62),(1.40±0.70),(0.21±0.05),(0.25±0.12),(0.26±0.11),(0.34±0.29),(0.74±0.59),and 0.25 h.The method is proved specific and repeatable and is suitable for the determination of psoralenoside,isopsoralenoside,calycosin-7-glucoside,ononin,pso-ralen,isopsoralen,methylnissolin,and neobavaisoflavone in the rat plasma,which can be applied to pharmacokinetic study.
作者
任慧
郭盛
张祎盈
李全
王恒斌
耿婉丽
尚尔鑫
钱大玮
段金廒
REN Hui;GUO Sheng;ZHANG Yi-ying;LI Quan;WANG Heng-bin;GENG Wan-li;SHANG Er-xin;QIAN Da-wei;DUAN Jin-ao(National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae,Nanjing University of Chinese Medicine,Nanjing 210023,China;Lei Yun Shang Pharmaceutical Group Co.,Ltd.,Suzhou 215003,China;Guangdong Leiyunshang Pharmaceutical Co.,Ltd.,Yunfu 527300,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2022年第1期215-223,共9页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81873189)
江苏省高校自然科学研究重大项目(18KJA360006)
江苏省“六大人才”高峰项目(YY-026)。
